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首页> 美国卫生研究院文献>Infection and Immunity >Minor Nucleotide Substitutions in the omp31 Gene of Brucella ovis Result in Antigenic Differences in the Major Outer Membrane Protein That It Encodes Compared to Those of the Other Brucella Species
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Minor Nucleotide Substitutions in the omp31 Gene of Brucella ovis Result in Antigenic Differences in the Major Outer Membrane Protein That It Encodes Compared to Those of the Other Brucella Species

机译：布鲁氏菌ovis的omp31基因中的小核苷酸取代导致编码的主要外膜蛋白与其他布鲁氏菌物种相比在抗原上的差异

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The gene coding for the major outer membrane protein Omp31 was sequenced in five Brucella species and their biovars. Although the omp31 genes appeared to be highly conserved in the genus Brucella, nine nucleotide substitutions were detected in the gene of Brucella ovis compared to that of Brucella melitensis. As shown by differential binding properties of monoclonal antibodies (MAbs) to the two Brucella species, these nucleotide substitutions result in different antigenic properties of Omp31. The antigenic differences were also evidenced when sera from B. ovis-infected rams were tested by Western blotting with the recombinant B. melitensis or B. ovis Omp31 proteins. Twelve available sera reacted with recombinant B. ovis Omp31, but only four of them reacted with recombinant B. melitensis Omp31. These results validate previous evidence for the potential of Omp31 as a diagnostic antigen for B. ovis infection in rams and demonstrate that B. ovis Omp31, instead of B. melitensis Omp31, should be used to evaluate this point. The antigenic differences between the B. melitensis and B. ovis Omp31 proteins should also be taken into account when Omp31 is evaluated as a candidate for the development of subcellular vaccines against B. ovis infection. No reactivity against recombinant B. melitensis Omp31 was detected, by Western blotting, with sera from B. melitensis-infected sheep. Accordingly, Omp31 does not seem to be a good diagnostic antigen for B. melitensis infections in sheep. Two immunodominant regions were identified on the B. ovis Omp31 protein by using recombinant DNA techniques and specific MAbs. Sera from B. ovis-infected rams that reacted with the recombinant protein were tested by Western blotting against one of these immunodominant regions shown to be exposed at the bacterial surface. Only 4 of the 12 sera reacted, but with strong intensity.
机译：在五个布鲁氏菌属及其生物变种中对主要外膜蛋白Omp31的编码基因进行了测序。尽管omp31基因在布鲁氏菌属中似乎是高度保守的，但是与布鲁氏菌相比，在布鲁氏菌属的基因中检测到九个核苷酸取代。如单克隆抗体（MAb）与两种布鲁氏菌属物种的结合特性不同所示，这些核苷酸取代导致Omp31的抗原特性不同。当用重组B. melitensis或B. ovis Omp31蛋白通过蛋白质印迹法检测来自B. ovis感染的公羊的血清时，也证明了抗原差异。十二种可利用的血清与重组B. ovis Omp31反应，但只有四个与重组B. melitensis Omp31反应。这些结果验证了Omp31作为公羊B. ovis感染诊断抗原的潜力的先前证据，并表明应使用B. ovis Omp31代替B. melitensis Omp31来评估这一点。当将Omp31评估为开发针对 B的亚细胞疫苗的候选药物时，也应考虑B. melitensis和B. ovis B. Omp31蛋白之间的抗原差异。 ovis 感染。对重组 B无反应性。用蛋白质印迹法检测了 B血清中的melitensis Omp31。感染了羊肉的绵羊。因此，Omp31似乎不是B的良好诊断抗原。绵羊感染了melitensis 。在 B上鉴定出两个免疫优势区域。 ovis Omp31蛋白，采用重组DNA技术和特异性单克隆抗体。 B的血清。通过Western印迹测试显示与细菌蛋白接触的这些免疫显性区域之一，从而检测与重组蛋白反应的经ovis感染的公羊。 12个血清中只有4个发生了反应，但强度很高。

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