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首页> 外文期刊>Infection and immunity >Toxicity of lipopolysaccharide and of soluble extracts of Salmonella typhimurium in mice immunized with a live attenuated aroA salmonella vaccine.
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Toxicity of lipopolysaccharide and of soluble extracts of Salmonella typhimurium in mice immunized with a live attenuated aroA salmonella vaccine.

机译：脂多糖和鼠伤寒沙门氏菌可溶性提取物在用减毒活aroA沙门氏菌疫苗免疫的小鼠中的毒性。

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Mice immunized intravenously 10 days earlier (but not those immunized 2 months earlier) with an attenuated Salmonella typhimurium SL3261 aroA live vaccine and tested for delayed-type hypersensitivity by injection of crude Salmonella extracts in the footpad can die within 24 to 48 h of an unexplained allergic reaction. The lethal reaction could be prevented by prior administration of anti-tumor necrosis factor alpha serum. Injection of lipopolysaccharide (LPS) (either purified phenol-water-extracted [Westphal] LPS or protein-rich trichloracetic acid-extracted [Boivin] LPS) was also lethal for mice immunized 10 days before. An LPS-rich crude Salmonella extract was more toxic than one which contained less LPS, suggesting that LPS may have been involved in the lethal reactions to crude antigens. Mild alkaline hydrolysis removes O-linked acyl groups from lipid A and eliminates many toxic effects of LPS; however, both Boivin LPS and Westphal LPS remained toxic for immunized mice after alkaline hydrolysis. In contrast, alkaline hydrolysis of crude whole Salmonella extracts (which caused marked protein degradation) reduced the lethal toxicity of the extracts, especially for an LPS-rich preparation. Mice immunized orally with the live vaccine did not show hypersensitivity to either LPS or crude extracts. The results suggest that the lethal reaction to crude Salmonella antigens in mice immunized 10 days earlier is complex, that tumor necrosis factor alpha is involved, and that allergic reactions to crude antigens (but not to LPS alone) can be reduced by mild alkaline hydrolysis.
机译：用减毒鼠伤寒沙门氏菌SL3261 aroA活疫苗进行静脉注射免疫的小鼠提前10天（但未接受2个月免疫的小鼠），通过在脚垫中注射粗制沙门氏菌提取物进行延迟型超敏反应测试，可能会在无法解释的情况下24至48小时内死亡过敏反应。事先给予抗肿瘤坏死因子α血清可预防致命反应。注射脂多糖（LPS）（纯化的酚水提取的[Westphal] LPS或富含蛋白质的三氯乙酸提取的[Boivin] LPS）也对10天前免疫的小鼠致死。富含LPS的粗沙门氏菌提取物比含有较少LPS的沙门氏菌提取物更具毒性，这表明LPS可能参与了对粗抗原的致死反应。轻度的碱水解可从脂质A去除O-连接的酰基，并消除LPS的许多毒性作用;然而，Boivin LPS和Westphal LPS对碱性水解后的免疫小鼠仍然具有毒性。相反，粗沙门氏菌粗提取物的碱水解（导致明显的蛋白质降解）降低了提取物的致死毒性，特别是对于富含LPS的制剂。经活疫苗口服免疫的小鼠对LPS或粗提物均未显示超敏反应。结果表明，在10天前免疫的小鼠中，对沙门氏菌抗原的致死反应很复杂，涉及肿瘤坏死因子α，通过温和的碱水解可以减少对粗抗原（而不是对LPS的过敏反应）的反应。

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