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首页> 外文期刊>Infection and immunity >Role of YadA in resistance to killing of Yersinia enterocolitica by antimicrobial polypeptides of human granulocytes.
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Role of YadA in resistance to killing of Yersinia enterocolitica by antimicrobial polypeptides of human granulocytes.

机译：YadA在抵抗人类粒细胞抗菌多肽杀死小肠结肠炎耶尔森菌中的作用。

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The virulence plasmid pYVe of Yersinia enterocolitica codes for the production of the outer membrane protein YadA and the secretion of several proteins, called Yops, which may protect this bacterium against killing by human granulocytes. Granulocytes kill ingested microorganisms by oxygen-dependent and oxygen-independent mechanisms, the latter including antimicrobial polypeptides. The aim of this study was to determine whether virulent (pYVe+) Y. enterocolitica and plasmid-cured avirulent (pYVe-) Y. enterocolitica differ in susceptibility to antimicrobial polypeptides extracted from granules of human granulocytes. The acetic acid granule extract contained several polypeptides with antimicrobial activity against Y. enterocolitica as determined by gel overlay and radial diffusion assays. Two of these polypeptides were identified as lysozyme and defensins. pYVe+ Y. enterocolitica was less susceptible than pYVe- Y. enterocolitica to the antimicrobial activity of granule extract, lysozyme, and defensins as determined in a suspension assay, which indicated that the pYVe plasmid mediates a reduced susceptibility to these polypeptides. The role of YadA in the resistance to antimicrobial polypeptides was analyzed by using mutants of Y. enterocolitica that specifically lack or express YadA. The results demonstrated that YadA conferred resistance to the killing of Y. enterocolitica by the granule extract. Together, these results indicate that the plasmid-encoded factor YadA contributes to the resistance of Y. enterocolitica to the killing by antimicrobial polypeptides of human granulocytes.
机译：小肠结肠炎耶尔森氏菌的致病性质粒pYVe编码外膜蛋白YadA的产生以及几种称为Yops的蛋白的分泌，这些蛋白可以保护该细菌免于被人类粒细胞杀死。粒细胞通过氧依赖性和氧依赖性机制杀死摄入的微生物，后者包括抗微生物多肽。这项研究的目的是确定毒性（pYVe +）肠炎耶尔森氏菌和质粒固化的无毒性（pYVe-）肠炎耶尔森氏菌对从人粒细胞颗粒提取的抗菌多肽的敏感性是否不同。乙酸颗粒提取物含有几种对小肠结肠炎耶尔森氏菌具有抗菌活性的多肽，如通过凝胶覆盖和径向扩散分析所测定。这些多肽中的两个被鉴定为溶菌酶和防御素。 pYVe +小肠结肠炎耶尔森氏菌比pYVe-小肠结肠炎耶尔森氏菌对悬浮液测定中的颗粒提取物，溶菌酶和防御素的抗菌活性更不敏感，这表明pYVe质粒介导了对这些多肽的敏感性降低。通过使用特异性缺乏或表达YadA的小肠结肠炎耶尔森氏菌突变体，分析了YadA在抗微生物多肽中的作用。结果表明，YadA赋予了对颗粒提取物杀死小肠结肠炎耶尔森氏菌的抗性。总之，这些结果表明，质粒编码的因子YadA有助于小肠结肠炎耶尔森氏菌对人粒细胞的抗菌多肽杀伤的抗性。

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