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首页> 外文期刊>Infection and immunity >Mycoplasma membrane lipoproteins induced proinflammatory cytokines by a mechanism distinct from that of lipopolysaccharide.
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Mycoplasma membrane lipoproteins induced proinflammatory cytokines by a mechanism distinct from that of lipopolysaccharide.

机译：支原体膜脂蛋白通过不同于脂多糖的机制诱导促炎细胞因子。

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To gain a clear understanding of the mechanisms by which mycoplasmas induced the expression of proinflammatory cytokines in monocytic cells, we have studied the induction of interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha, and IL-6 by mycoplasmas in three distinct human myelomonocytic cell lines in comparison with induction by lipopolysaccharide (LPS). HL-60 cell line did not release cytokines when induced with either LPS or mycoplasmas. In contrast to LPS, mycoplasmas failed to increase the weak levels of tumor necrosis factor alpha secreted by phorbol myristate acetate-differentiated U937 cells. In addition, Northern (RNA) blot analysis of cytokine expression in these cells showed that the induction of IL-1 beta by mycoplasmas involves, unlike that by LPS, posttranscriptional events. Interestingly, in THP-1 cells, cytokine induction pathways triggered by mycoplasmas remained operational under conditions where LPS pathways were abolished, suggesting functional independence. The study of cytokine-inducing activity displayed by distinct fractions derived from a series of different mycoplasma species demonstrated that lipid membrane constituents were largely responsible for these effects. Finally, we have demonstrated that tyrosine phosphorylation is a crucial event in the mycoplasma-mediated induction of proinflammatory cytokines in either THP-1 cells or human monocytes.
机译：为了清楚了解支原体诱导单核细胞表达促炎细胞因子的机制，我们研究了支原体在白细胞介素1β（IL-1 beta），肿瘤坏死因子α和IL-6的诱导作用。与脂多糖（LPS）诱导相比，三种不同的人类骨髓单核细胞系。当用LPS或支原体诱导时，HL-60细胞系不释放细胞因子。与LPS相反，支原体不能增加佛波肉豆蔻酸酯乙酸酯分化的U937细胞分泌的肿瘤坏死因子α的弱水平。另外，对这些细胞中细胞因子表达的Northern（RNA）印迹分析表明，与LPS不同，支原体诱导IL-1β涉及转录后事件。有趣的是，在THP-1细胞中，由支原体触发的细胞因子诱导途径在LPS途径被废除的条件下仍然有效，表明其功能独立性。对源自一系列不同支原体物种的不同部分显示的细胞因子诱导活性的研究表明，脂质膜成分在很大程度上起着这些作用。最后，我们证明了酪氨酸磷酸化是支原体介导的THP-1细胞或人类单核细胞促炎细胞因子诱导中的关键事件。

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