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首页> 外文期刊>Infection and immunity >Antigenic characterization and analysis of the human immune response to outer membrane protein E of Branhamella catarrhalis.
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Antigenic characterization and analysis of the human immune response to outer membrane protein E of Branhamella catarrhalis.

机译：人类对卡他布氏杆菌外膜蛋白E的免疫应答的抗原表征和分析。

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摘要

Outer membrane protein E (OMP E) is a 50-kDa major OMP of Branhamella catarrhalis. Polyclonal antisera and four monoclonal antibodies (MAbs) to OMP E were generated to study its antigenic structure. All antibodies recognized epitopes in all 19 B. catarrhalis strains tested by immunoblot assays. By flow cytometry, it was determined that MAbs 1B3 and 9G10d recognized epitopes which are expressed on the surface of the intact bacterium, while MAbs IC11 and 7C10 recognized epitopes which were buried within the outer membrane. A competitive enzyme-linked immunosorbent assay showed that MAbs 1B3 and 9G10d recognize the same or closely related epitopes. Proteinase K treatment of whole bacterial cells revealed that MAbs 1B3 and 9G10d recognize a surface-exposed epitope located in the 17-kDa region towards the amino terminus of OMP E. The human serum and mucosal antibody responses to OMP E in adults with chronic bronchitis were studied. A majority of these patients had immunoglobulin A to OMP E in sputum supernatants. None of ten adults who experienced lower respiratory tract infections due to B. catarrhalis demonstrated a clear-cut rise in antibody titer to OMP E in serum or sputum supernatant. This study has demonstrated that OMP E has at least one surface-exposed epitope which is highly conserved among strains of B. catarrhalis and which is located in the amino-terminal 184 amino acids of the molecule.
机译：外膜蛋白E（OMP E）是卡他氏杆菌的50 kDa主要OMP。产生了多克隆抗血清和四种针对OMP E的单克隆抗体（MAb），以研究其抗原结构。通过免疫印迹测定法测试的所有19种卡他芽孢杆菌菌株中所有抗体均识别表位。通过流式细胞术确定MAb 1B3和9G10d识别在完整细菌表面表达的表位，而MAb IC11和7C10识别被埋在外膜内的表位。竞争性酶联免疫吸附试验表明，单克隆抗体1B3和9G10d识别相同或紧密相关的表位。对整个细菌细胞进行蛋白酶K处理后发现，单克隆抗体1B3和9G10d识别位于OMP E氨基末端17-kDa区的表面暴露表位。慢性支气管炎成人的人血清和粘膜抗体对OMP E的反应是研究。这些患者大多数在痰上清液中具有针对OMP E的免疫球蛋白A。十名因卡他芽孢杆菌而导致下呼吸道感染的成年人中，没有一个人的血清或痰上清液中针对OMP E的抗体滴度明显增加。这项研究表明，OMP E具有至少一个表面暴露的抗原决定簇，该抗原决定簇在卡他芽孢杆菌菌株中高度保守，并且位于该分子的氨基末端184个氨基酸中。

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