...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Surface-exposed and antigenically conserved determinants of outer membrane proteins of Branhamella catarrhalis.
【24h】

Surface-exposed and antigenically conserved determinants of outer membrane proteins of Branhamella catarrhalis.

机译：卡那氏杆菌外膜蛋白的表面暴露和抗原决定簇。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The outer membrane proteins (OMPs) of Branhamella catarrhalis were studied in an effort to identify surface-exposed determinants that are conserved among strains of the bacterium. Aliquots of polyclonal antiserum were absorbed individually by strains of B. catarrhalis. The absorbed antisera were tested in comparison with unabsorbed antiserum in an immunoblot assay against OMPs of the homologous strain. The absence of a band recognized by antibodies in the absorbed antiserum compared with the unabsorbed antiserum indicated that surface-exposed determinants of the absorbing strain cross-reacted with determinants on the homologous strain. Two antisera were absorbed individually by 20 strains of B. catarrhalis, and the absorbed sera were studied in this way in immunoblot assays. OMP E (molecular weight, ca. 56,000) expresses surface-exposed determinants that are shared among 17 of the 20 strains studied. Antibodies to OMP G (molecular weight, 28,000) were absorbed from both antisera by 14 of the 20 strains. These studies demonstrate that OMP E and OMP G express determinants that are exposed on the surface of the intact bacterium. Furthermore, these determinants are antigenically conserved among a majority of strains of B. catarrhalis. On the basis of these observations, OMPs E and G should be considered when bacterial antigens are evaluated as potential vaccine candidates.
机译：研究了卡他氏杆菌的外膜蛋白（OMP），以鉴定在细菌菌株中保守的表面暴露的决定簇。多克隆抗血清的等分试样被卡他双歧杆菌单独吸收。在针对同源菌株OMP的免疫印迹试验中，将吸收的抗血清与未吸收的抗血清进行比较。与未吸收的抗血清相比，吸收的抗血清中不存在抗体识别的条带，表明吸收菌株的表面暴露的决定簇与同源菌株上的决定簇发生交叉反应。两种抗血清分别被20株粘膜炎双歧杆菌吸收，并通过免疫印迹法对吸收的血清进行了研究。 OMP E（分子量，约56,000）表达表面暴露的决定簇，在研究的20个菌株中共有17个。 20种菌株中有14种从两种抗血清中吸收了OMP G抗体（分子量为28,000）。这些研究表明OMP E和OMP G表达了暴露于完整细菌表面的决定簇。此外，这些决定簇在大多数卡他芽胞杆菌菌株中在抗原上是保守的。基于这些观察，当细菌抗原被评估为潜在的疫苗候选者时，应考虑OMPs E和G。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号