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首页> 外文期刊>Infection and immunity >Pseudomonas aeruginosa lasRTranscription Correlates with the Transcription of lasA,lasB, and toxA in Chronic Lung Infections Associated with Cystic Fibrosis
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Pseudomonas aeruginosa lasRTranscription Correlates with the Transcription of lasA,lasB, and toxA in Chronic Lung Infections Associated with Cystic Fibrosis

机译：铜绿假单胞菌lasRT转录与囊性纤维化相关的慢性肺部感染中lasA，lasB和toxA的转录相关

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The role of Pseudomonas aeruginosa quorum-sensing systems in the lung infections associated with cystic fibrosis (CF) has not been examined. The purpose of this study was to determine if genes regulated by the LasR-LasI quorum-sensing system were coordinately regulated by the P. aeruginosa populations during the lung infections associated with CF. We also wanted to ascertain if there was a relationship between the expression of lasR, a transcriptional regulator, and some P. aeruginosa virulence factors during these infections. We extracted RNAs from the bacterial populations of 131 sputa taken from 23 CF patients. These RNAs were blotted and hybridized with probes to P. aeruginosa lasA,lasB, and toxA. The hybridization signals from each probe were ranked, and the rankings were analyzed by a Spearman rank correlation to determine if there was an association between the population transcript accumulations for the three genes. The correlations between the transcript accumulation patterns of pairs of the genes suggested that lasA, lasB, andtoxA might be coordinately regulated during CF lung infections. To determine if this coordinate regulation might be due to regulation by LasR, we probed RNAs, extracted from 84 sputa, with thelasR, lasA, lasB, toxA, and algD probes. Statistical analysis indicated thatlasR transcript accumulation correlated tolasA, lasB, toxA, andalgD transcript accumulations. These results indicated thatlasR may at least partially regulate or be coordinately regulated with lasA, lasB, toxA, and algD during the lung infections associated with CF. These results also suggested that the LasR-LasI quorum-sensing system may control the expression of at least some virulence factors in the lungs of patients with CF.
机译：尚未研究铜绿假单胞菌群体感应系统在与囊性纤维化（CF）相关的肺部感染中的作用。这项研究的目的是确定由LasR-LasI群体感应系统调控的基因是否由 P协调调控。与CF相关的肺部感染期间的铜绿菌种群我们还想确定转录调节子 lasR 的表达与某些 P之间是否存在关联。这些感染过程中的铜绿毒力因子。我们从23名CF患者的131个痰中提取了RNA。这些RNA被印迹并与探针杂交至 P。铜绿lasA ， lasB 和 toxA 。对来自每个探针的杂交信号进行排名，并通过Spearman排名相关性对排名进行分析，以确定三个基因的群体转录本积累之间是否存在关联。这些基因对的转录本积累模式之间的相关性表明，在CF肺部感染期间，可能会协同调节 lasA ， lasB 和 toxA 。为了确定这种协调调控是否可能是由于LasR调控引起的，我们用 lasR ， lasA ， lasB ， toxA 和 algD 探针。统计分析表明， lasR 转录本的积累与 lasA ， lasB ， toxA 和 algD相关成绩单积累。这些结果表明， lasR 可能至少部分地受 lasA ， lasB ， toxA 和与CF相关的肺部感染中的 algD 。这些结果还表明，LasR-LasI群体感应系统可以控制CF患者肺中至少一些毒力因子的表达。

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