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首页> 外文期刊>Infection and immunity >Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate.
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Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate.

机译：人颌下舌下唾液促进白色念珠菌与聚甲基丙烯酸甲酯的粘附。

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The purpose of this study was to identify components of saliva that interact with Candida albicans in solution and that may modulate adhesion to dental acrylic (polymethylmethacrylate [PMMA]) surfaces. Saliva-derived pellicles extracted from C. albicans blastoconidia and hyphal-form cells mixed with fresh human submandibular-sublingual saliva (HSMSL) contained predominantly high- and low-molecular-weight mucins (MG1 and MG2, respectively). In contrast, few components from fresh human parotid saliva were adsorbed to yeast cells. Coating PMMA beads with HSMSL significantly enhanced (10-fold) adhesion of both growth forms of C. albicans compared with human parotid saliva (2-fold), suggesting a role for mucins in adhesion. HSMSL-enhanced adhesion was completely abolished by preadsorbing HSMSL with either blastoconidia or hyphal-form cells prior to coating PMMA. However, coating PMMA with purified salivary mucins or the addition of mucin to preadsorbed saliva did not enhance or restore adhesion to levels found with fresh HSMSL. Adhesion assays employing guanidine-treated fresh HSMSL showed a complete lack of Candida binding, suggesting that subjecting HSMSL to dissociating conditions may alter a property of salivary mucins crucial for C. albicans adhesion. Protease and glycosidase treatment of yeast cells significantly reduced adhesion to HSMSL-coated PMMA. In addition, preincubation of C. albicans with mannose and galactose inhibited adhesion to HSMSL-coated PMMA. These results suggest that mucins may play a role in C. albicans adhesion to saliva-coated PMMA and that a glycoprotein on the yeast surface may be involved in these events.
机译：这项研究的目的是确定唾液中与白色念珠菌相互作用的唾液成分，这些成分可能会调节与牙科用丙烯酸（聚甲基丙烯酸甲酯[PMMA]）表面的粘附力。从白色念珠菌和与新鲜人下颌下舌下唾液（HSMSL）混合的菌丝状细胞中提取的唾液来源的防护膜主要含有高分子量和低分子量的粘蛋白（分别为MG1和MG2）。相反，新鲜的人腮腺唾液中几乎没有成分被吸附到酵母细胞中。与人腮腺唾液相比，涂有HSMSL的PMMA珠与人腮腺唾液相比（2倍）显着提高了白色念珠菌的两种生长形式的粘附力（10倍），表明粘蛋白在粘附中的作用。在包被PMMA之前，通过将胚芽孢杆菌或菌丝状细胞预吸附HSMSL可以完全消除HSMSL增强的粘附力。但是，用纯化的唾液粘蛋白​​包被PMMA或在预吸附的唾液中添加粘蛋白并不能将粘附力提高或恢复到新鲜HSMSL所发现的水平。采用胍处理的新鲜HSMSL进行的粘附测定表明完全缺乏念珠菌结合，这表明使HSMSL处于解离条件可能会改变唾液粘蛋白​​的特性，而唾液粘蛋白​​的特性对白色念珠菌的粘附至关重要。蛋白酶和糖苷酶处理酵母细胞可显着降低与HSMSL涂层PMMA的粘附力。另外，白色念珠菌与甘露糖和半乳糖的预孵育抑制了对HSMSL涂层PMMA的粘附。这些结果表明粘蛋白可能在白色念珠菌对唾液包被的PMMA的粘附中起作用，并且酵母表面上的糖蛋白可能参与了这些事件。

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