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首页> 外文期刊>Infection and immunity >Intracellular multiplication and toxic destruction of cultured macrophages by Capnocytophaga canimorsus.
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Intracellular multiplication and toxic destruction of cultured macrophages by Capnocytophaga canimorsus.

机译：Capnocytophaga canimorsus对培养的巨噬细胞的细胞内增殖和毒性破坏。

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摘要

Capnocytophaga canimorsus is a gram-negative rod that causes opportunistic infections resulting in bacteremia, septicemia, meningitis, and death in immunocompromised, splenectomized, and alcoholic individuals. Infections caused by a related species, Capnocytophaga cynodegmi, remain localized at the site of the wound where the organism is introduced. Both organisms are part of the normal canine oral flora and are introduced through puncture wounds via dog bites. We found that both C. canimorsus and C. cynodegmi attach, are phagocytized, and multiply intracellularly in J774 mouse macrophage cells. After 48 h of infection by C. canimorsus, large sections of the macrophage cell layer were observed to detach and lyse, while the monolayer infected with C. cynodegmi demonstrated no cytotoxic effects. Tissue culture supernatants from the C. canimorsus-infected J774 cells filtered through a 0.22-micron-pore membrane produced a similar effect on fresh monolayers, while filtrates from C. cynodegmi and uninfected controls produced no effect. No endotoxin release was observed in these supernatants. We conclude that the cytotoxic phenotype of C. canimorsus is the likely result of a toxin produced by this organism.
机译：Capnocytophaga canimorsus是一种革兰氏阴性杆菌，可引起机会性感染，导致免疫力低下，脾脏切除和酒精中毒的人发生菌血症，败血病，脑膜炎和死亡。由相关物种Capnocytophaga cynodegmi引起的感染仍定位在引入生物的伤口部位。这两种生物都是正常犬口腔菌群的一部分，并通过狗咬伤的穿刺伤口引入。我们发现C. canimorsus和C. cynodegmi都被吞噬，并在J774小鼠巨噬细胞中在细胞内繁殖。在被犬念珠菌感染48小时后，观察到大部分巨噬细胞层发生分离和裂解，而被单胞藻感染的单层细胞没有细胞毒性作用。来自经0.22微米孔膜过滤的犬念珠菌感染的J774细胞的组织培养上清液对新鲜单层细胞产生相似的作用，而来自食蟹梭菌和未感染对照的滤液则没有作用。在这些上清液中未观察到内毒素释放。我们得出结论，C。canimorsus的细胞毒性表型可能是这种生物体产生的毒素的结果。

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