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首页> 外文期刊>Infection and immunity >Hemagglutination and proteoglycan binding by the Lyme disease spirochete, Borrelia burgdorferi.
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Hemagglutination and proteoglycan binding by the Lyme disease spirochete, Borrelia burgdorferi.

机译：莱姆病螺旋体伯氏疏螺旋体的血凝和蛋白聚糖结合。

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The ability of the Lyme disease spirochete to attach to host components may contribute to its ability to infect diverse tissues. We present evidence that the Lyme disease spirochete expresses a lectin activity that promotes agglutination of erythrocytes and bacterial attachment to glycosaminoglycans. Among a diverse collection of 21 strains of Lyme disease spirochete, hemagglutinating activity was easily detected in all but 3 strains, and these three strains were noninfectious. The ability to agglutinate erythrocytes was associated with the ability of the spirochete to bind to the sulfated polysaccharide dextran sulfate and to mammalian cells. Soluble dextran sulfate was a potent inhibitor of both hemagglutination and attachment to mammalian cells, while dextran had no effect on either activity, suggesting that dextran sulfate may inhibit attachment by mimicking host cell glycosaminoglycans. Consistent with this, the spirochete bound to immobilized heparin, and soluble heparin inhibited bacterial adhesion to mammalian cells. The bacterium did not bind efficiently to Vero cells treated with heparinase or heparitinase or to mutant CHO cell lines that are deficient in proteoglycan synthesis. Sulfation of glycosaminoglycans was critical for efficient bacterial recognition, as Vero cells treated with an inhibitor of sulfation, or a mutant CHO cell line that produces undersulfated heparan sulfate, did not mediate maximal spirochetal binding. Binding of the spirochete to extracellular matrix also appeared to be dependent upon this attachment pathway. These findings suggest that a glycosaminoglycan-binding activity which can be detected by hemagglutination contributes to the attachment of the Lyme disease spirochete to host cells and matrix.
机译：莱姆病螺旋体附着于宿主成分的能力可能有助于其感染多种组织的能力。我们目前的证据表明，莱姆病螺旋体表达一种凝集素活性，可促进红细胞的凝集和细菌对糖胺聚糖的附着。在21种莱姆病螺旋体菌株的多样化集合中，除3种菌株外，其他所有菌株中均易于检测到血凝活性，而这3种菌株均无感染性。凝集红细胞的能力与螺旋体结合硫酸化多糖葡聚糖硫酸盐和哺乳动物细胞的能力有关。可溶性硫酸葡聚糖是一种有效的血凝反应和附着于哺乳动物细胞的抑制剂，而葡聚糖对这两种活性均没有影响，表明硫酸葡聚糖可以通过模仿宿主细胞的糖胺聚糖来抑制附着。与此一致，螺环与固定的肝素结合，而可溶性肝素则抑制细菌与哺乳动物细胞的粘附。该细菌无法有效结合用肝素酶或肝素酶处理的Vero细胞或蛋白聚糖合成不足的突变CHO细胞系。糖胺聚糖的硫酸化对于细菌的有效识别至关重要，因为用硫酸化抑制剂处理的Vero细胞或产生硫酸亚硫酸乙酰肝素的突变CHO细胞系不会介导最大的螺旋形结合。螺旋体与细胞外基质的结合似乎也依赖于该附着途径。这些发现表明，可以通过血凝检测到的糖胺聚糖结合活性有助于莱姆病螺旋体附着到宿主细胞和基质上。

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