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首页> 外文期刊>Infection and immunity >Target organs of infection in guinea pigs with acquired congenital syphilis.
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Target organs of infection in guinea pigs with acquired congenital syphilis.

机译：获得性先天性梅毒的豚鼠感染的目标器官。

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The target organs of infection in guinea pigs with asymptomatic acquired or congenital syphilis were identified by PCR and in some cases by rabbit infectivity test (RIT). The prevalence of Treponema pallidum DNA was examined in the following seven organs: the inguinal and mesenteric lymph nodes, spleen, liver, kidney, heart, and brain. Test samples consisted of 95 organs from two genetically different strains of female guinea pigs (C4-deficient and Albany) with different susceptibilities to cutaneous infection by T. pallidum and 195 organs from their asymptomatic offspring. Twenty organs from dams of both strains injected with heat-killed T. pallidum and 19 organs from their progeny served as negative controls. The infections of mothers and neonates were documented by PCR, RIT, and serology. Though any of the organs tested could be infected, there was a spirochetal predilection for some anatomical locations, such as the lymph nodes, heart, and brain, regardless of the strain, route of maternal infection, and age. None of the 49 organs collected from control animals were positive by PCR. In infected C4-deficient dams, one to four organs were positive by PCR, whereas the organs of 7 of their 27 (25%) asymptomatic offspring were treponemal DNA negative, despite evidence of immunoglobulin M treponemal antibodies. Comparative analysis done by both PCR and RIT on a limited number of samples showed 90% agreement between results. An examination of multiple samples obtained from single organs demonstrated that even within 24 h of spirochetemia, when most organs appeared to be infected, not all samples from an individual organ were positive by PCR. A specific immunological response in guinea pigs with congenital syphilis was a more consistent parameter of vertical transmission than was an analysis of T. pallidum DNA.
机译：通过PCR鉴定无症状获得性或先天性梅毒的豚鼠的目标感染器官，在某些情况下通过兔传染性试验（RIT）进行鉴定。在以下七个器官中检查了梅毒螺旋体DNA的患病率：腹股沟和肠系膜淋巴结，脾脏，肝脏，肾脏，心脏和大脑。测试样品由来自两种遗传不同的雌性豚鼠（C4缺陷型和奥尔巴尼）品系的95个器官组成，它们对苍白螺旋体皮肤感染的敏感性不同，并且来自无症状后代的195个器官。注射了热灭活的梅毒螺旋体的两个菌株的大坝的二十个器官和后代的十九个器官用作阴性对照。 PCR，RIT和血清学记录了母亲和新生儿的感染情况。尽管测试的任何器官都可能被感染，但某些解剖位置（例如淋巴结，心脏和大脑）有螺旋状的倾向，而不管应变，孕产妇感染的途径和年龄如何。通过PCR从对照动物收集的49个器官中没有一个是阳性的。在受感染的C4缺陷大坝中，通过PCR可以检测到1-4个器官为阳性，而其27个无症状后代中有7个（占25％）的器官呈螺旋体DNA阴性，尽管有免疫球蛋白M呈螺旋体抗体的证据。通过PCR和RIT对有限数量的样品进行的比较分析显示，结果之间有90％的一致性。对从单个器官获得的多个样品进行的检查表明，即使在螺旋藻病的24小时内，当大多数器官似乎都被感染时，并非单个器官的所有样品都通过PCR呈阳性。先天性梅毒的豚鼠的特异性免疫反应是垂直传播的参数，比梅毒螺旋体DNA的分析更一致。

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