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首页> 外文期刊>Infection and immunity >Pasteurella multocida toxin is a mitogen for bone cells in primary culture.
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Pasteurella multocida toxin is a mitogen for bone cells in primary culture.

机译：多杀性巴斯德氏菌毒素是原代培养中骨细胞的促分裂原。

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摘要

The effect of recombinant Pasteurella multocida toxin (PMT) on primary cultures of embryonic chick bone-derived osteoblastic cells was investigated. It was found that PMT was a potent mitogen for primary derived chicken osteoblasts. The toxin stimulated DNA synthesis and cell proliferation in quiescent osteoblasts at the first passage and accelerated cell growth in subconfluent cultures. Cell viability was not affected by PMT, even at relatively high concentrations. Osteoblast numbers increased in a dose-dependent manner in response to PMT. Intracellular inositol phosphates were elevated in response to PMT, but no elevation in cyclic AMP (cAMP) levels was evident. Indeed, PMT inhibited cAMP elevation in osteoblasts in response to cholera toxin at a stage before other PMT-mediated events take place. In addition to increased cell turnover, PMT down-regulated the expression of several markers of osteoblast differentiation. Both alkaline phosphatase and type I collagen were reduced, but osteonectin was not affected. The in vitro deposition of mineral in cultures of primary osteoblasts and osteoblast-like osteosarcoma cells was also inhibited by the presence of PMT. This suggests that PMT interferes with differentiation at a preosteoblastic stage.
机译：研究了重组败血巴斯德氏菌毒素（PMT）对胚胎鸡骨来源成骨细胞的原代培养的影响。发现PMT是原代鸡成骨细胞的有效促分裂原。该毒素在第一个传代时刺激了静态成骨细胞中的DNA合成和细胞增殖，并在次融合培养物中加速了细胞生长。即使在相对较高的浓度下，细胞存活率也不受PMT影响。响应PMT，成骨细胞数量以剂量依赖性方式增加。细胞内肌醇磷酸盐响应PMT升高，但没有明显增加环AMP（cAMP）水平。实际上，在其他PMT介导的事件发生之前的一个阶段，PMT抑制了霍乱毒素对成骨细胞中cAMP的升高。除了增加细胞更新，PMT还下调了成骨细胞分化的几种标志物的表达。碱性磷酸酶和I型胶原均减少，但骨连接蛋白不受影响。 PMT的存在也抑制了原发性成骨细胞和成骨细胞样骨肉瘤细胞培养物中矿物质的体外沉积。这表明PMT在成骨前期阶段干扰分化。

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