• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Staphylococcus aureus binding to human nasal mucin.
【24h】

Staphylococcus aureus binding to human nasal mucin.

机译：金黄色葡萄球菌与人鼻粘蛋白结合。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Colonization of human nasal mucosa with Staphylococcus aureus sets the stage for subsequent systemic infection. This study characterizes S. aureus adhesion to nasal mucosa in vitro and investigates the interaction of S. aureus with human nasal mucin. S. aureus binding to cell-associated and cell-free mucus was greater than to nonmucin-coated epithelial cells. Scanning electron microscopy of S. aureus incubated with human nasal mucosal tissue showed minimal binding to ciliated respiratory epithelium. In a solid-phase assay, S. aureus bound to purified human nasal mucin-coated wells significantly more than to bovine serum albumin-coated microtiter wells. Binding to mucin was saturable in a dose- and time-dependent fashion. Staphylococcal adherence to human nasal mucin was inhibited by bovine submaxillary mucin but not by fibrinogen. Pretreatment of mucin with periodate but not with pronase reduced adherence. Trypsin treatment of the bacteria significantly reduced adherence to mucin. 125I-labelled nasal mucin bound to two surface proteins (138 and 127 kDa) of lysostaphin-solubilized S. aureus. Binding to human nasal mucin occurs in part via specific adhesin-receptor interactions involving bacterial proteins and the carbohydrate moiety in mucin. These experiments suggest that S. aureus binding to mucin may be critical for colonization of the nasopharyngeal mucosa.
机译：金黄色葡萄球菌在人鼻粘膜上的定殖为随后的全身感染奠定了基础。该研究表征了金黄色葡萄球菌在体外对鼻粘膜的粘附，并研究了金黄色葡萄球菌与人鼻粘蛋白的相互作用。金黄色葡萄球菌对细胞相关和无细胞粘液的结合大于对非粘蛋白包被的上皮细胞的结合。与人鼻粘膜组织一起孵育的金黄色葡萄球菌的扫描电子显微镜显示与纤毛呼吸上皮的结合最小。在固相测定中，金黄色葡萄球菌与纯化的人鼻粘蛋白包被的孔的结合比与牛血清白蛋白包被的微量滴定的孔的结合要多得多。与粘蛋白的结合以剂量和时间依赖性方式饱和。葡萄球菌对人鼻粘蛋白的粘附被牛颌下粘蛋白抑制，但未被纤维蛋白原抑制。用高碘酸盐而不是用链霉蛋白酶预处理粘蛋白会降低粘连。胰蛋白酶对细菌的处理显着降低了对粘蛋白的粘附。 125I标记的鼻粘蛋白与溶葡萄球菌素增溶的金黄色葡萄球菌的两个表面蛋白（138和127 kDa）结合。与人鼻粘蛋白的结合部分地通过涉及细菌蛋白和粘蛋白中碳水化合物部分的特定粘附素-受体相互作用而发生。这些实验表明，金黄色葡萄球菌与粘蛋白的结合对于鼻咽粘膜的定植可能至关重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号