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首页> 外文期刊>Infection and immunity >Tonsillar application of killed Streptococcus mutans induces specific antibodies in rabbit saliva and blood plasma without inducing a cross-reacting antibody to human cardiac muscle.
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Tonsillar application of killed Streptococcus mutans induces specific antibodies in rabbit saliva and blood plasma without inducing a cross-reacting antibody to human cardiac muscle.

机译：扁桃体被杀灭后的变形链球菌的扁桃体应用可在兔唾液和血浆中诱导特异性抗体，而不会诱导针对人心肌的交叉反应抗体。

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When Streptococcus mutans cells are injected into the skeletal muscle of rabbits, an antibody against human cardiac muscle, as well as an anti-S. mutans antibody, is induced in blood plasma. Our previous study showed that when sheep erythrocytes are applied to palatine tonsils, an antibody against the applied cells is induced both in blood plasma and saliva. This antibody has no activity against cardiac muscle. It is not clear, however, if S. mutans application to the tonsils evokes an antibody response against cardiac muscle. In this study, we immunized rabbits against S. mutans or Streptococcus sobrinus by tonsillar application or by intramuscular injection every 3 days for 6 weeks. Tonsillar applications of formalin-killed cells of S. mutans induced saliva immunoglobulin A (IgA) and blood plasma IgG to the applied cells. In contrast, intramuscular injection of such cells induced only blood plasma IgG. When the route of immunization was intramuscular injection, antibodies in blood plasma cross-reacted with cardiac muscle. By enzyme-immunohistochemistry and Ouchterlony immunodiffusion tests, no cross-reaction to cardiac muscle was observed with the antibody in saliva or in blood plasma after the tonsillar applications. Western blotting of the S. mutans antigen showed that blood plasma from rabbits injected with S. mutans reacted with antigens of 46, 52, 62, and 85 kDa, while that from rabbits subjected to tonsillar application of S. mutans did not react with these bands. Similar results were obtained for S. sobrinus applications. Thus, tonsillar applications of mutants group streptococci induce antibodies differing in antigen specificity and do not induce any cross-reacting antibody to cardiac muscle.
机译：当将变形链球菌细胞注射到兔子的骨骼肌中时，会产生针对人心肌的抗体以及抗S抗体。变形抗体，在血浆中被诱导。我们以前的研究表明，当将绵羊红细胞应用于p扁桃体时，血浆和唾液中均会诱导出针对所应用细胞的抗体。该抗体对心肌没有活性。但是，尚不清楚是否将扁桃链球菌应用于扁桃体会引起针对心肌的抗体反应。在这项研究中，我们通过扁桃体应用或每3天肌肉注射6周来免疫兔抗变形链球菌或sobrinus链球菌。变形链球菌福尔马林杀死的细胞的扁桃体应用可诱导唾液免疫球蛋白A（IgA）和血浆IgG进入所应用的细胞。相反，肌肉注射这类细胞仅诱导血浆IgG。当免疫途径是肌内注射时，血浆中的抗体会与心肌发生交叉反应。通过酶免疫组织化学和Ouchterlony免疫扩散试验，扁桃体应用后，唾液或血浆中未观察到抗体与心肌的交叉反应。变形链球菌抗原的Western印迹显示，注射变形链球菌的兔子的血浆与46、52、62和85 kDa的抗原发生反应，而扁桃体应用扁桃体链球菌的兔子的血浆没有反应乐队。对于S. sobrinus应用获得了相似的结果。因此，突变体组链球菌的扁桃体应用诱导了抗原特异性不同的抗体，并且不诱导针对心肌的任何交叉反应抗体。

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