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首页> 外文期刊>Infection and immunity >Molecular Characterization of a Haemaphysalis longicornis Tick Salivary Gland-Associated 29-Kilodalton Protein and Its Effect as a Vaccine against Tick Infestation in Rabbits
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Molecular Characterization of a Haemaphysalis longicornis Tick Salivary Gland-Associated 29-Kilodalton Protein and Its Effect as a Vaccine against Tick Infestation in Rabbits

机译：血红蛋白T虱唾液腺相关29-千达尔顿蛋白的分子表征及其对T虱侵袭的预防作用

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The use of tick vaccines in mammalian hosts has been shown to be the most promising alternative tick control method to current use of acaricides, which suffers from a number of limitations. However, the success of this method is dependent on the identification, cloning, and in vitro expression of tick molecules involved in the mediation of key physiological roles with respect to the biological success of a tick as a vector and pest. We have sequenced and characterized aHaemaphysalis longicornis tick salivary gland-associated cDNA coding for a 29-kDa extracellular matrix-like protein. This protein is expressed in both unfed and fed immature and mature H. longicornis ticks. The predicted amino acid sequence of p29 shows high homology to sequences of some known extracellular matrix like-proteins with the structural conservation similar to all known collagen proteins. Immunization with the recombinant p29 conferred a significant protective immunity in rabbits, resulting in reduced engorgement weight for adult ticks and up to 40 and 56% mortality in larvae and nymphs that fed on the immunized rabbits. We speculate that this protein is associated with formation of tick cement, a chemical compound that enables the tick to remain attached to the host, and suggest a role for p29 as a candidate tick vaccine molecule for the control of ticks. We have discussed our findings with respect to the search of tick molecules for vaccine candidates.
机译：已经证明，在哺乳动物宿主中使用壁虱疫苗是目前使用杀螨剂的最有希望的替代壁虱控制方法，这种方法存在许多局限性。但是，这种方法的成功取决于of的分子的鉴定，克隆和体外表达，这些分子参与了有关physiological作为载体和害虫的生物学成功的关键生理作用的介导。我们已经测序和表征了 Haemaphysalis longicornis 壁虱唾液腺相关的cDNA，编码29 kDa的细胞外基质样蛋白。该蛋白质在未进食和进食的未成熟和成熟H中均表达。滴答滴答声。 p29的预测氨基酸序列与某些已知细胞外基质类似蛋白的序列具有高度同源性，其结构保守性类似于所有已知胶原蛋白。重组p29的免疫赋予兔子显着的保护性免疫力，导致成年s的吞噬体重降低，以免疫兔子为食的幼虫和若虫的死亡率分别降低40％和56％。我们推测该蛋白与壁虱胶的形成有关，该化合物使壁虱能够保持与宿主的附着，并暗示p29作为壁虱控制候选壁虱疫苗分子的作用。我们已经讨论了有关寻找候选疫苗的壁虱分子的发现。

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