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首页> 外文期刊>Infection and immunity >Burkholderia thailandensis as a Model System for the Study of the Virulence-Associated Type III Secretion System of Burkholderia pseudomallei
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Burkholderia thailandensis as a Model System for the Study of the Virulence-Associated Type III Secretion System of Burkholderia pseudomallei

机译：伯克霍尔德氏菌作为模型系统研究假性伯克霍尔德氏菌的毒力相关的III型分泌系统

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Burkholderia pseudomallei is a bacterial pathogen that causes a broad spectrum of clinical symptoms collectively known as melioidosis. Since it can be acquired by inhalation and is difficult to eradicate due to its resistance to a wide group of antibiotics and capacity for latency, work with B. pseudomallei requires a biosafety level 3 (BSL-3) containment facility. The bsa (Burkholderia secretion apparatus)-encoded type III secretion system (TTSS) has been shown to be required for its full virulence in a number of animal models. TTSSs are export devices found in a variety of gram-negative bacteria that translocate bacterial effector proteins across host cell membranes into the cytoplasm of host cells. Although the Bsa TTSS has been shown to play an important role in the ability of B. pseudomallei to survive and replicate in mammalian cells, escape from the endocytic vacuole, and spread from cell to cell, little is known about its effectors mediating these functions. Using bioinformatics, we identified homologs of several known TTSS effectors from other bacteria in the B. pseudomallei genome. In addition, we show that orthologs of these putative effectors exist in the genome of B. thailandensis, a closely related bacterium that is rarely pathogenic to humans. By generating a Bsa TTSS mutant B. thailandensis strain, we also demonstrated that the Bsa TTSS has similar functions in the two species. Therefore, we propose B. thailandensis as a useful BSL-1 model system to study the role of the Bsa TTSS during Burkholderia infection of mammalian cells and animals.
机译：假伯克霍尔德氏菌是一种细菌病原体，可引起广泛的临床症状，统称为类胡突病。由于它可以通过吸入获得，并且由于其对多种抗生素的抵抗力和潜伏期而难以根除，因此可以与 B一起使用。 pseudomallei 需要3级生物安全（BSL-3）围堵设施。已经证明， bsa （伯克霍尔德氏菌分泌物 a pparatus）编码的III型分泌系统（TTSS）才能完全发挥其毒力。许多动物模型。 TTSS是在多种革兰氏阴性细菌中发现的输出设备，这些细菌可将细菌效应蛋白跨宿主细胞膜转运到宿主细胞的细胞质中。尽管已显示Bsa TTSS在 B的能力中起重要作用。 pseudomallei 能够在哺乳动物细胞中存活和复制，从胞吞液泡中逃逸，并在细胞之间扩散，因此对于其介导这些功能的效应器知之甚少。使用生物信息学，我们从 B中的其他细菌中鉴定了几种已知的TTSS效应子的同源物。假基因组。此外，我们表明这些推定的效应子的直系同源物存在于 B的基因组中。 thailandensis ，一种密切相关的细菌，对人类几乎没有致病性。通过产生Bsa TTSS突变体 B。 thailandensis 菌株，我们还证明了Bsa TTSS在这两个物种中具有相似的功能。因此，我们提出 B。 thailandensis 是一种有用的BSL-1模型系统，用于研究Bsa TTSS在伯克霍尔德菌感染哺乳动物细胞和动物中的作用。

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