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首页> 外文期刊>Journal of Clinical Microbiology >Identification and Discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes
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Identification and Discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes

机译:实时PCR靶向III型分泌系统基因的鉴定和鉴别Burkholderia pseudomallei,B. mallei和B. thailandensis

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摘要

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.
机译:假伯克霍尔德氏菌 B。 Mallei 是两种高度致病的细菌,分别负责类鼻oid病和腺体病。两者密切相关,也可以误认为 B。 thailandensis ,一种非致病性物种。为了提高它们的差异鉴定,我们描述了一种基于水解探针的实时PCR方法,使用了这三个物种之间III型分泌系统基因的不均匀分布。

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