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Characterization of a Unique ClpB Protein of Mycoplasma pneumoniae and Its Impact on Growth

机译：肺炎支原体独特的ClpB蛋白的表征及其对生长的影响

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Mycoplasma pneumoniae accounts for 20 to 30% of all community-acquired pneumonia and has been associated with other airway pathologies, including asthma, and a range of extrapulmonary manifestations. Although the entire genomic sequence of M. pneumoniae has been completed, the functions of many of these genes in mycoplasma physiology are unknown. In this study, we focused on clpB, a well-known heat shock gene in other bacteria, to examine its role in mycoplasma growth. Transcriptional and translational analyses of heat shock in M. pneumoniae indicated that clpB is significantly upregulated, reinforcing its status as a critical responder to heat stress. Interestingly, M. pneumoniae ClpB does not use dual translational start points for ClpB synthesis, like other ClpB-characterized bacteria. Biochemical characterization of purified M. pneumoniae recombinant ClpB revealed casein- and lysine-independent ATPase activity and DnaK-DnaJ-GrpE-dependent chaperone activity. An M. pneumoniae mini-Tn4001-integrated, clpB-null mutant was impaired in its ability to replicate under permissive growth conditions, demonstrating the growth-promoting status of ClpB.
机译：肺炎支原体占所有社区获得性肺炎的20％至30％，并与其他呼吸道疾病（包括哮喘）和一系列肺外表现有关。虽然 M的整个基因组序列。肺炎已经完成，其中许多基因在支原体生理中的功能尚不清楚。在这项研究中，我们集中于 clpB （一种在其他细菌中众所周知的热休克基因）来研究其在支原体生长中的作用。转录和翻译分析中的热休克。肺炎表明 clpB 明显上调，增强了其作为热应激的关键响应者的地位。有趣的是， M。与其他以ClpB为特征的细菌一样，肺炎ClpB不使用双重翻译起点进行ClpB合成。纯化的 M的生化特性。肺炎支原体重组ClpB显示酪蛋白和赖氨酸独立的ATPase活性和DnaK-DnaJ-GrpE依赖的伴侣活性。一个 M。肺炎 mini-Tn 4001 整合的 clpB -null突变体在允许的生长条件下的复制能力受到损害，证明了ClpB的生长促进状态。

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