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首页> 外文期刊>Infection and immunity >Humoral Immunity through Immunoglobulin M Protects Mice from an Experimental Actinomycetoma Infection by Nocardia brasiliensis
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Humoral Immunity through Immunoglobulin M Protects Mice from an Experimental Actinomycetoma Infection by Nocardia brasiliensis

机译：通过免疫球蛋白M的体液免疫保护小鼠免受巴西诺卡氏菌的实验放线菌感染。

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An experimental model of infection with Nocardia brasiliensis, used as an example of a facultative intracellular pathogen, was tested. N. brasiliensis was injected into the rear foot pads of BALB/c mice to establish an infection. Within 30 days, infected animals developed a chronic actinomycetoma infection. Batch cultures of N. brasiliensis were used to purify P61, P38, and P24 antigens; P61 is a catalase, and P38 is a protease with strong caseinolytic activity. Active and passive immunizations of BALB/c mice with these three purified soluble antigens were studied. Protection was demonstrated for actively immunized mice. However, immunity lasted only 30 days. Other groups of immunized mice were bled at different times, and their sera were passively transferred to naive recipients that were then infected with N. brasiliensis. Sera collected 5, 6, and 7 days after donor immunization conferred complete, long-lasting protection. The protective effect of passive immunity decreased when sera were collected 2 weeks after donor immunization. However, neither the early sera (1-, 2-, and 3-day sera) nor the later sera (30- or 45-day sera) prevented the infection. Hyperimmune sera with the highest levels of immunoglobulin G (IgG) to N. brasiliensis antigens did not protect at all. The antigens tested induced two IgM peaks. The first peak was present 3 days after immunization but was not antigen specific and did not transfer protection. The second peak was evident 7 days after immunization, was an IgM response, was antigen specific, and conferred protection. This results clearly demonstrate that IgM antibodies protect the host against a facultative intracellular bacterium.
机译：测试了以巴西诺卡氏菌感染的实验模型，该模型是兼性细胞内病原体的一个例子。 N。将巴西利亚注射到BALB / c小鼠的后足垫中以建立感染。在30天内，被感染的动物出现了慢性放线菌感染。 N的分批培养。巴西的用于纯化P61，P38和P24抗原。 P61为过氧化氢酶，P38为具有强酪蛋白水解活性的蛋白酶。研究了这三种纯化的可溶性抗原对BALB / c小鼠的主动和被动免疫。已证明对主动免疫的小鼠具有保护作用。但是，免疫只持续了30天。其他组免疫小鼠在不同时间采血，并将其血清被动转移至幼稚受体，然后再感染 N。巴西利亚。捐献者免疫后第5、6和7天收集的血清可提供完整，持久的保护。供体免疫2周后收集血清时，被动免疫的保护作用降低。但是，早期血清（1天，2天和3天血清）或后期血清（30天或45天血清）均不能预防感染。免疫球蛋白G（IgG）最高水平的超免疫血清。巴西利亚抗原根本没有保护。测试的抗原诱导了两个IgM峰。第一个峰出现在免疫后3天，但不是抗原特异性的，没有转移保护作用。免疫后7天出现第二个峰，这是IgM反应，是抗原特异性的，并具有保护作用。该结果清楚地表明，IgM抗体保护宿主抵抗兼性细胞内细菌。

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