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首页> 外文期刊>Infection and immunity >Effect of Corticosteroids and Neuropeptides on the Expression of Defensins in Bovine Tracheal Epithelial Cells
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Effect of Corticosteroids and Neuropeptides on the Expression of Defensins in Bovine Tracheal Epithelial Cells

机译：皮质类固醇和神经肽对牛气管上皮细胞防御素表达的影响

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Susceptibility to bacterial pneumonia in cattle is enhanced by stressors such as transportation, weaning, and commingling, which trigger a physiologic stress response resulting in elevated levels of endogenous corticosteroids and catecholamines. To determine the effect of neuroendocrine mediators on the expression of innate defense peptides in the lung, bovine tracheal epithelial cells were exposed to dexamethasone, catecholamines, acetylcholine, or substance P, and then β-defensin expression was quantified using real-time reverse transcription-PCR. Basal expression of tracheal antimicrobial peptide (TAP) mRNA was not affected by any of the mediators tested. However, induction of TAP expression by lipopolysaccharide was significantly inhibited by pretreatment with dexamethasone. Bronchial biopsy specimens from dexamethasone-treated calves had significantly lower expression of TAP and lingual antimicrobial peptide (LAP) mRNA than saline-treated controls following 48 h of treatment. Lipopolysaccharide-elicited neutrophil recruitment was enhanced in the lungs of dexamethasone-treated calves compared to saline-treated controls. These findings indicate that modulation of epithelial antimicrobial peptide expression is one mechanism through which corticosteroids and stress may impair innate pulmonary defenses.
机译：运输，断奶和混合等应激因素会增强牛对细菌性肺炎的易感性，这些应激因素会触发生理应激反应，导致内源性皮质类固醇和儿茶酚胺水平升高。为了确定神经内分泌介质对肺中先天防御肽表达的影响，将牛气管上皮细胞暴露于地塞米松，儿茶酚胺，乙酰胆碱或P物质，然后使用实时逆转录定量β-防御素的表达， PCR。气管抗菌肽（TAP）mRNA的基础表达不受任何测试介体的影响。但是，地塞米松预处理可显着抑制脂多糖对TAP表达的诱导。处理48小时后，地塞米松治疗的小腿支气管活检标本的TAP和舌头抗菌肽（LAP）mRNA的表达明显低于生理盐水处理的对照组。与盐水处理的对照组相比，地塞米松处理的犊牛肺中脂多糖引起的中性粒细胞募集得到增强。这些发现表明，上皮抗微生物肽表达的调节是皮质类固醇和压力可能削弱先天性肺部防御的一种机制。

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