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首页> 外文期刊>Infection and immunity >Salmonella Pathogenicity Island 4-Mediated Adhesion Is Coregulated with Invasion Genes in Salmonella enterica
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Salmonella Pathogenicity Island 4-Mediated Adhesion Is Coregulated with Invasion Genes in Salmonella enterica

机译：沙门氏菌致病岛4介导的粘附与肠沙门氏菌的入侵基因。

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Salmonella pathogenicity island 4 (SPI4) encodes a type I secretion system and the cognate substrate protein, SiiE. We have recently demonstrated that SiiE is a giant nonfimbrial adhesin involved in the adhesion of Salmonella enterica serovar Typhimurium to polarized epithelial cells. We also observed that under in vitro culture conditions, the synthesis and secretion of SiiE coincided with the activation of Salmonella invasion genes. These observations prompted us to investigate the regulation of SPI4 genes in detail. A novel approach for the generation of reporter gene fusions was employed to generate single-copy chromosomal fusions to various genes within SPI4, and the expression of these fusions was investigated. We analyzed the regulation of SPI4 genes and the roles of various regulatory systems for SPI4 expression. Our data show that the expression of SPI4 genes is coregulated with SPI1 invasion genes by the global regulator SirA. Expression of a SPI4 gene was also reduced in the absence of HilA, the central local regulator of SPI1 gene expression. Both SirA and HilA functions were required for the secretion of SiiE and the SPI4-mediated adhesion. Our data demonstrate that SPI4-mediated adhesion, as well as SPI1-mediated invasion, are tightly coregulated by the same regulatory circuits and induced under similar environmental conditions.
机译：沙门氏菌致病岛4（SPI4）编码I型分泌系统和相关底物蛋白SiiE。我们最近证明，SiiE是一种巨大的非纤维粘附素，参与了 Salmonella enterica 鼠伤寒鼠伤寒沙门氏菌与极化上皮细胞的粘附。我们还观察到，在体外培养条件下，SiiE的合成和分泌与沙门氏菌入侵基因的激活相吻合。这些发现促使我们详细研究了SPI4基因的调控。生成报告基因基因融合体的一种新方法被用来生成与SPI4中各种基因的单拷贝染色体融合体，并研究了这些融合体的表达。我们分析了SPI4基因的调控以及SPI4表达的各种调控系统的作用。我们的数据表明，通过全球监管机构SirA，SPI4基因的表达与SPI1入侵基因紧密相关。在不存在SPI1基因表达的中央局部调节子HilA的情况下，SPI4基因的表达也降低了。 SiiE的分泌和SPI4介导的粘附都需要SirA和HilA功能。我们的数据表明，SPI4介导的粘附以及SPI1介导的入侵被相同的调节电路紧密结合，并在相似的环境条件下被诱导。

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