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首页> 外文期刊>Infection and immunity >Proteome and Antigen Profiling of Coxiella burnetii Developmental Forms
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Proteome and Antigen Profiling of Coxiella burnetii Developmental Forms

机译：伯氏Coxiella发育形式的蛋白质组学和抗原谱分析

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A biphasic developmental cycle whereby highly resistant small-cell variants (SCVs) are generated from large-cell variants (LCVs) is considered fundamental to the virulence of Coxiella burnetii, the causative agent of human Q fever. In this study a proteome analysis of C. burnetii developmental forms was conducted to provide insight into their unique biological and immunological properties. Silver-stained gels of SCV and LCV lysates separated by two-dimensional (2-D) gel electrophoresis resolved over 675 proteins in both developmental forms. Forty-eight proteins were greater than twofold more abundant in LCVs than in SCVs, with six proteins greater than twofold more abundant in SCVs than in LCVs. Four and 15 upregulated proteins of SCVs and LCVs, respectively, were identified by mass spectrometry, and their predicted functional roles are consistent with a metabolically active LCV and a structurally resistant SCV. One-dimensional and 2-D immunoblots of cell form lysates probed with sera from infected/vaccinated guinea pigs and convalescent-phase serum from human patients who had recovered from acute Q fever, respectively, revealed both unique SCV/LCV antigens and common SCV/LCV antigens that were often differentially synthesized. Antigens recognized during human infection were identified by mass spectroscopy and included both previously described immunodominant proteins of C. burnetii and novel immunogenic proteins that may be important in the pathophysiology of clinical Q fever and/or the induction of protective immunity.
机译：从大细胞变异体（LCV）产生高抗性的小细胞变异体（SCV）的双相发展周期被认为是人类Q发热的致病性毒素（Coxiella burnetii ）毒力的基础。在这项研究中，对 C进行蛋白质组分析。进行了burnetii 发育形式的研究，以了解其独特的生物学和免疫学性质。通过二维（2-D）凝胶电泳分离的SCV和LCV裂解液的银染凝胶分离出两种发育形式的675种蛋白质。 LCV中48种蛋白质的丰度比SCV中的蛋白质高两倍，而SCV中六种蛋白质的丰度是LCV中的两倍以上。质谱分别鉴定了SCV和LCV的4种和15种上调蛋白，它们的预测功能与代谢活性LCV和结构抗性SCV一致。从感染/接种豚鼠的血清中检测到的细胞形式裂解物的一维和二维免疫印迹，以及从急性Q发热中恢复的人类患者的恢复期血清，分别显示出独特的SCV / LCV抗原和常见的SCV /通常差异合成的LCV抗原。通过质谱鉴定在人类感染期间识别的抗原，并且包括先前描述的 C的免疫优势蛋白。 Burnetii 和新型免疫原性蛋白，可能在临床Q发热和/或诱导保护性免疫的病理生理中很重要。

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