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首页> 外文期刊>Infection and immunity >Intralesional Regulatory T-Cell Suppressive Function during Human Acute and Chronic Cutaneous Leishmaniasis Due to Leishmania guyanensis
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Intralesional Regulatory T-Cell Suppressive Function during Human Acute and Chronic Cutaneous Leishmaniasis Due to Leishmania guyanensis

机译：在因利什曼原虫引起的人类急性和慢性皮肤利什曼病中的内部调节性T细胞抑制功能

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The levels of regulatory T cells (Treg cells), analyzed by Foxp3 mRNA expression, were determined in lesions from patients with acute cutaneous leishmaniasis (ACL) and chronic cutaneous leishmaniasis (CCL). We demonstrated that Treg cells preferentially accumulate in lesions from ACL patients during the early phase of infection (lesion duration of less than 1 month). In addition, levels of Foxp3 mRNA transcripts were significantly higher in specimens from patients with CCL than in those from patients with ACL, suggesting a critical role of intralesional Treg cells in CCL. Intralesional Treg cells from both ACL and CCL patients were shown to have suppressive functions in vitro, since they inhibited the gamma interferon (IFN-γ) produced by CD4+ CD25? T cells purified from peripheral blood mononuclear cells from the same patient in response to Leishmania guyanensis stimulation. Intralesional 2,3-indoleamine dioxygenase (IDO) mRNA expression was associated with that of Foxp3, suggesting a role for IDO in the suppressive activity of intralesional Treg cells. In addition, a role, albeit minor, of interleukin-10 (IL-10) was also demonstrated, since neutralization of IL-10 produced by intralesional T cells increased IFN-γ production by effector cells in an in vitro suppressive assay. These results confirm the role of intralesional Treg cells in the immunopathogenesis of human Leishmania infection, particularly in CCL patients.
机译：在患有急性皮肤利什曼病（ACL）和慢性皮肤利什曼病（CCL）的患者的病变中测定通过Foxp3 mRNA表达分析的调节性T细胞（Treg细胞）的水平。我们证明，在感染的早期阶段（病变持续时间少于1个月），Treg细胞优先在ACL患者的病变中蓄积。此外，CCL患者的标本中Foxp3 mRNA转录水平显着高于ACL患者的标本，表明病灶内Treg细胞在CCL中起关键作用。 ACL和CCL患者的肠内Treg细胞均具有抑制作用，因为它们抑制CD4 + CD25 ？ T产生的γ-干扰素（IFN-γ）。从同一患者的外周血单核细胞中纯化的细胞对Leemmania Guyanensis 刺激产生反应。肠内2,3-吲哚胺双加氧酶（IDO）mRNA表达与Foxp3相关，表明IDO在损伤内Treg细胞抑制活性中的作用。另外，还证实了白细胞介素10（IL-10）的作用，尽管是次要的，因为在体外抑制测定中，病灶内T细胞产生的IL-10的中和增加了效应细胞产生的IFN-γ产生。这些结果证实了病变内Treg细胞在人利什曼原虫感染的免疫发病机制中的作用，特别是在CCL患者中。

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