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首页> 外文期刊>Infection and immunity >Comparison of the Regulation, Metabolic Functions, and Roles in Virulence of the Glyceraldehyde-3-Phosphate Dehydrogenase Homologues gapA and gapB in Staphylococcus aureus
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Comparison of the Regulation, Metabolic Functions, and Roles in Virulence of the Glyceraldehyde-3-Phosphate Dehydrogenase Homologues gapA and gapB in Staphylococcus aureus

机译：金黄色葡萄球菌中甘油醛-3-磷酸脱氢酶同源物gapA和gapB的调节，代谢功能及其在毒力中的作用比较

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The Gram-positive bacterium Staphylococcus aureus contains two glyceraldehyde-3-phosphate dehydrogenase (GAPDH) homologues known as GapA and GapB. GapA has been characterized as a functional GAPDH protein, but currently there is no biological evidence for the role of GapB in metabolism in S. aureus. In this study we show through a number of complementary methods that S. aureus GapA is essential for glycolysis while GapB is essential in gluconeogenesis. These proteins are reciprocally regulated in response to glucose concentrations, and both are influenced by the glycolysis regulator protein GapR, which is the first demonstration of the role of this regulator in S. aureus and the first indication that GapR homologues control genes other than those within the glycolytic operon. Furthermore, we show that both GapA and GapB are important in the pathogenesis of S. aureus in a Galleria mellonella model of infection, showing for the first time in any bacteria that both glycolysis and gluconeogenesis have important roles in virulence.
机译：革兰氏阳性金黄色葡萄球菌含有两个称为GapA和GapB的甘油三磷酸三磷酸脱氢酶（GAPDH）同源物。 GapA被表征为功能性GAPDH蛋白，但是目前尚无生物学证据证明GapB在金黄色葡萄球菌的代谢中的作用。在这项研究中，我们通过许多补充方法表明，金黄色葡萄球菌GapA对于糖酵解必不可少，而GapB在糖异生中必不可少。这些蛋白质响应葡萄糖浓度而相互调节，并且均受糖酵解调节蛋白GapR的影响，这是该调节剂在金黄色葡萄球菌中的作用的第一个证明，也是第一个表明GapR同源物控制除基因之外的基因的迹象。糖酵解操纵子。此外，我们显示在Galleria mellonella感染模型中，GapA和GapB在金黄色葡萄球菌的发病机理中均很重要，这首次表明在任何细菌中糖酵解和糖异生均在毒力中具有重要作用。

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