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首页> 外文期刊>Infection and immunity >Local Host Response to Chlamydial Urethral Infection in Male Guinea Pigs
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Local Host Response to Chlamydial Urethral Infection in Male Guinea Pigs

机译：本地宿主对雄性豚鼠衣原体尿道感染的反应

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Very little is known about the host response to chlamydial genital infection in the male, particularly about the nature of the local response in the urethra. In this study, the pathological and immunologic responses to urethral infection of the male guinea pig with Chlamydia caviae (Chlamydophila caviae) were characterized both during a primary infection and following a challenge infection. A dose-response experiment found that the 50% infectious dose for male urethral infection was 78 inclusion-forming units. The histopathologic response was similar to that of the female, with an initial acute inflammatory response followed by a chronic inflammatory response and plasma cell infiltration. Production of IgG and IgA antibodies in local urethral secretions developed following infection, and levels of both increased in a typical anamnestic response following a challenge infection. CD4 and CD8 T cells, as well as B cells, were observed in the local site by flow cytometry, with a slightly increased number of CD8 cells. Following challenge infection, the dominant anamnestic response was solely in the B-cell compartment, with only a minimal number of T cells. The T-cell response was clearly a Th1 response, as judged by increased levels of gamma interferon (IFN-γ), interleukin-12 p40 (IL-12p40), and IL-2. The proinflammatory cytokines and chemokines IL-8, IL-1β, tumor necrosis factor alpha (TNF-α), CCL2 (monocyte chemoattractant protein 1 [MCP-1]), and CCL5 (RANTES) were elicited in the urethra following primary infection, but only CCL5 showed increased levels upon challenge. This study represents the first comprehensive analysis of the local immune response in the male urethra to a chlamydial genital infection.
机译：关于宿主对男性衣原体生殖器感染的反应知之甚少，特别是对尿道局部反应的性质了解甚少。在这项研究中，表征了在初次感染期间和攻击性感染后，对雄性豚鼠豚鼠衣原体（Chlamydophila caviae）尿道感染的病理学和免疫学反应。剂量反应实验发现，男性尿道感染的50％感染剂量为78个包涵体形成单位。组织病理学反应与女性相似，先是急性炎症反应，然后是慢性炎症反应和浆细胞浸润。感染后局部尿道分泌物中产生IgG和IgA抗体，攻击感染后典型的记忆消除反应中两者的水平均升高。通过流式细胞术在局部部位观察到CD4和CD8 T细胞以及B细胞，CD8细胞的数量略有增加。激发感染后，主要的记忆消除反应仅存在于B细胞区室中，只有极少量的T细胞。根据γ干扰素（IFN-γ），白介素12 p40（IL-12p40）和IL-2水平的升高判断，T细胞反应显然是Th1反应。原发感染后在尿道中诱发促炎细胞因子和趋化因子IL-8，IL-1β，肿瘤坏死因子α（TNF-α），CCL2（单核细胞趋化蛋白1 [MCP-1]）和CCL5（RANTES），但只有CCL5在激发后显示出升高的水平。这项研究代表了男性尿道中对衣原体生殖器感染的局部免疫反应的首次综合分析。

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