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Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

机译：空肠弯曲杆菌NCTC11168对禽胃肠道的高水平定植的适应

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The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that this strain is a poor colonizer. Experimental colonization and persistence studies were carried out with C. jejuni NCTC11168 by using 2-week-old Light Sussex chickens possessing an acquired natural gut flora. After inoculation, NCTC11168 initially colonized the intestine poorly. However, after 5 weeks we observed adaptation to high-level colonization, which was maintained after in vitro passage. The adapted strain exhibited greatly increased motility. A second strain, C. jejuni 11168H, which had been selected under in vitro conditions for increased motility (A. V. Karlyshev, D. Linton, N. A. Gregson, and B. W. Wren, Microbiology >148:473-480, 2002), also showed high-level intestinal colonization. The levels of colonization were equivalent to those of six other strains, assessed under the same conditions. There were four mutations in C. jejuni 11168H that reduced colonization; maf5, flaA (motility and flagellation), and kpsM (capsule deficiency) eliminated colonization, whereas pglH (general glycosylation system deficient) reduced but did not eliminate colonization. This study showed that there was colonization of the avian intestinal tract by a Campylobacter strain having a known genome sequence, and it provides a model for colonization and persistence studies with specific mutations.
机译：最近已经确定了人类病原体空肠弯曲杆菌NCTC11168的基因组序列，但是由于报道了该菌株是弱定居者，因此对鸡的定殖和持久性研究受到限制。用 C进行了实验定植和持久性研究。通过使用具有获得的天然肠道菌群的两周大的轻型苏塞克斯鸡来空肠NCTC11168。接种后，NCTC11168最初在肠道中的定位较差。但是，在5周后，我们观察到了对高水平定植的适应，该定植在体外传代后得以维持。适应的菌株表现出极大的运动性。第二株， C。空肠菌11168H，它是在体外条件下选择的，以提高运动能力（AV Karlyshev，D。Linton，NA Gregson和BW Wren，微生物学，<148> 473-480，2002年），还表现出高水平的肠道定植。在相同条件下评估的定殖水平与其他六个菌株的定殖水平相同。在 C中有四个突变。空肠 11168H减少了定植; maf5 ， flaA （运动性和鞭毛）和 kpsM （胶囊缺乏）消除了定植，而 pglH （一般）糖基化系统缺陷）减少但没有消除定植。这项研究表明，具有已知基因组序列的 lopybacter 菌株在禽肠道中存在定殖，并且它为特定突变的定殖和持久性研究提供了模型。

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