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首页> 外文期刊>Infection and immunity >Strain Variation in Glycosaminoglycan Recognition Influences Cell-Type-Specific Binding by Lyme Disease Spirochetes
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Strain Variation in Glycosaminoglycan Recognition Influences Cell-Type-Specific Binding by Lyme Disease Spirochetes

机译：糖胺聚糖识别中的菌株变异影响莱姆病螺旋体对细胞类型的特异性结合。

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Lyme disease, a chronic multisystemic disorder that can affect the skin, heart, joints, and nervous system is caused by Borrelia burgdorferi sensu lato. Lyme disease spirochetes were previously shown to bind glycosaminoglycans (GAGs). In the current study, the GAG-binding properties of eight Lyme disease strains were determined. Binding by two high-passage HB19 derivatives to Vero cells could not be inhibited by enzymatic removal of GAGs or by the addition of exogenous GAG. The other six strains, which included a different high-passage HB19 derivative (HB19 clone 1), were shown to recognize both heparan sulfate and dermatan sulfate in cell-binding assays, but the relative efficiency of binding to these two GAGs varied among the strains. Strains N40, CA20-2A, and PBi bound predominantly to heparan sulfate, PBo bound both heparan sulfate and dermatan sulfate roughly equally, and VS461 and HB19 clone 1 recognized primarily dermatan sulfate. Cell binding by strain HB19 clone 1 was inhibited better by exogenous dermatan sulfate than by heparin, whereas heparin was the better inhibitor of binding by strain N40. The GAG-binding preference of a Lyme disease strain was reflected in its cell-type-specific binding. Strains that recognized predominantly heparan sulfate bound efficiently to both C6 glioma cells and EA-Hy926 cells, whereas strains that recognized predominantly dermatan sulfate bound well only to the glial cells. The effect of lyase treatment of these cells on bacterial binding was consistent with the model that cell-type-specific binding was a reflection of the GAG-binding preference. We conclude that the GAG-binding preference varies with the strain of Lyme disease spirochete and that this variation influences cell-type-specific binding in vitro.
机译：莱姆病是一种可能影响皮肤，心脏，关节和神经系统的慢性多系统疾病，是由 Borrelia burgdorferi sensu lato引起的。先前显示莱姆病螺旋体会结合糖胺聚糖（GAG）。在当前的研究中，确定了八种莱姆病菌株的GAG结合特性。通过酶促去除GAG或添加外源GAG不能抑制两种高通量HB19衍生物与Vero细胞的结合。在细胞结合试验中，其他六种菌株（包括不同的高通量HB19衍生物（HB19克隆1））可识别硫酸乙酰肝素和硫酸皮肤素，但结合这两种GAG的相对效率因菌株而异。菌株N40，CA20-2A和PBi主要与硫酸乙酰肝素结合，PBo大致相等地结合硫酸乙酰肝素和硫酸皮肤素，并且VS461和HB19克隆1主要识别硫酸皮肤素。 HB19克隆1菌株的细胞结合受到外源硫酸皮肤素的抑制作用要好于肝素，而肝素是N40菌株的结合抑制剂。莱姆病菌株的GAG结合偏好反映在其细胞类型特异性结合中。主要识别硫酸乙酰肝素的菌株与C6胶质瘤细胞和EA-Hy926细胞均有效结合，而主要识别硫酸皮肤素的菌株仅与神经胶质细胞结合良好。这些细胞的裂解酶处理对细菌结合的影响与细胞类型特异性结合是GAG结合偏好的反映的模型一致。我们得出结论，GAG结合偏好随莱姆病螺旋体的菌株而变化，并且这种变化影响体外细胞类型特异性结合。

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