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Receptor-Dependent Immune Responses in Pigs after Oral Immunization with F4 Fimbriae

机译：F4菌毛口服免疫后，猪的受体依赖性免疫反应

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F4 receptor-positive (F4R+) and F4 receptor-negative (F4R?) pigs were orally vaccinated with purified F4 fimbriae of enterotoxigenic Escherichia coli (ETEC). Serum immunoglobulin G (IgG) and IgA responses were readily detected in F4R+ animals, whereas immune responses were not detected in F4R? animals. Even after a subsequent oral infection with virulent F4+ ETEC and a booster immunization with F4, the F4R? animals remained F4 seronegative whereas the unvaccinated F4R+ pigs exhibited clear IgA and IgG responses. These results clearly demonstrate that F4Rs are a prerequisite for an immune response following oral immunization. Furthermore, indications that oral F4 vaccination can induce mucosal protection were obtained, since the experimental ETEC infection did not induce a systemic booster response or fecal ETEC excretion in orally vaccinated F4R+ pigs, in contrast to the clear immune response and ETEC excretion of unvaccinated F4R+ animals. F4-specific IgA antibodies could be found in the feces of the vaccinated F4R+ pigs. They are secreted at the intestinal mucosal surface and appear to prevent ETEC infection. The F4R-dependent induction of a mucosal immune response can be used as a model to better understand mucosal immunization and mucosal immune responses and can contribute to the development of oral vaccines in veterinary as well as in human medicine.
机译：F4受体阳性（F4R + ）和F4受体阴性（F4R ？ （ETEC）。在F4R + 动物中很容易检测到血清免疫球蛋白G（IgG）和IgA反应，而在F4R ？动物中未检测到免疫反应。即使随后用强力F4 + ETEC口服感染并用F4加强免疫后，F4R ？动物仍保持F4血清阴性，而未接种疫苗的F4R + 猪表现出明显的IgA和IgG反应。这些结果清楚地表明，F4Rs是口服免疫后免疫应答的先决条件。此外，获得了表明口服F4疫苗可以诱导粘膜保护的迹象，因为与透明免疫相比，实验性ETEC感染在口服疫苗的F4R + 猪中没有诱导全身加强反应或粪便ETEC排泄。未接种疫苗的F4R + 动物的免疫应答和ETEC排泄。在接种的F4R + 猪的粪便中发现了F4特异性IgA抗体。它们分泌在肠粘膜表面，似乎可以预防ETEC感染。 F4R依赖性的粘膜免疫应答诱导可以用作更好地了解粘膜免疫和粘膜免疫应答的模型，并且可以促进兽用和人类医学中口服疫苗的开发。

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