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首页> 外文期刊>Infection and immunity >Purification and Properties of Proline-Rich Antimicrobial Peptides from Sheep and Goat Leukocytes
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Purification and Properties of Proline-Rich Antimicrobial Peptides from Sheep and Goat Leukocytes

机译：绵羊和山羊白细胞中富含脯氨酸的抗菌肽的纯化和性质

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We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5α and ChBac5, to detailed analysis. OaBac5α and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. While the peptides remained active againstEscherichia coli, Pseudomonas aeruginosa,Bacillus subtilis, and Listeria monocytogenesin 100 mM NaCl, they lost activity against Staphylococcus aureus and Candida albicans under these conditions. ChBac5 was shown to bind lipopolysaccharide, a property that could enhance its ability to kill gram-negative bacteria. Proline-rich Bac5 peptides are highly conserved in ruminants and may contribute significantly to their innate host defense mechanisms.
机译：我们从用弹性蛋白酶处理过的绵羊和山羊白细胞提取物中纯化了三种富含脯氨酸的抗菌肽，并对其中的两个OaBac5α和ChBac5进行了详细分析。 OaBac5α和ChBac5彼此同源，与牛Bac5同源。两者在低浓度盐条件下均显示出有效的广谱抗菌活性。虽然这些肽在100 mM NaCl中对大肠杆菌，铜绿假单胞菌，枯草芽孢杆菌和单核细胞增生李斯特菌保持活性在这些条件下，它们失去了对金黄色葡萄球菌和白色念珠菌的活性。 ChBac5被证明与脂多糖结合，该特性可以增强其杀死革兰氏阴性细菌的能力。富含脯氨酸的Bac5肽在反刍动物中高度保守，并且可能对其先天宿主防御机制起重要作用。

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