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首页> 外文期刊>Infection and immunity >SFS, a Novel Fibronectin-Binding Protein from Streptococcus equi, Inhibits the Binding between Fibronectin and Collagen
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SFS, a Novel Fibronectin-Binding Protein from Streptococcus equi, Inhibits the Binding between Fibronectin and Collagen

机译：SFS，一种来自马链球菌的新型纤连蛋白结合蛋白，可抑制纤连蛋白与胶原蛋白之间的结合

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The obligate parasitic bacterium Streptococcus equisubsp. equi is the causative agent of strangles, a serious disease of the upper respiratory tract in horses. In this study we have, using shotgun phage display, cloned from S. equisubsp. equi and characterized a gene, calledsfs, encoding a protein termed SFS, representing a new type of fibronectin (Fn)-binding protein. The sfs gene was found to be present in all 50 isolates of S. equi subsp.equi tested and in 41 of 48 S. equi subsp.zooepidemicus isolates tested. The sfs gene is down-regulated during growth in vitro compared to fnz, a previously characterized gene encoding an Fn-binding protein fromS. equi subsp. zooepidemicus. Sequence comparisons revealed no similarities to previously characterized Fn-binding proteins, but high scores were obtained against collagen. Besides similarity due to the high content of glycine, serine, and proline residues present in both proteins, there was a nine-residue motif present both in collagen and in the Fn-binding domain of SFS. By searching the Oklahoma S. pyogenes database, we found that this motif is also present in a potential cell surface protein fromS. pyogenes. Protein SFS was found to inhibit the binding between Fn and collagen in a concentration-dependent way.
机译：专性寄生细菌马链球菌亚种。 equi 是勒死的病原，勒死是马的上呼吸道严重疾病。在这项研究中，我们使用散弹枪噬菌体展示从 S克隆了该克隆。 equi subsp。 equi 并鉴定了一个名为 sfs 的基因，该基因编码一种称为SFS的蛋白，代表一种新型的纤连蛋白（Fn）结合蛋白。发现 sfs 基因存在于 S的所有50个分离株中。 equi subsp。 equi 进行了测试，并在48个 S中有41个。 equi 亚种 zooepidemicus 分离株。与 fnz 相比， sfs 基因在体外生长过程中被下调，后者是以前表征的编码来自 S的Fn结合蛋白的基因。 equi 子空间 zooepidemicus 。序列比较显示与以前表征的Fn结合蛋白没有相似性，但是针对胶原蛋白获得了高分。除了由于两种蛋白质中存在的甘氨酸，丝氨酸和脯氨酸残基含量高而具有相似性外，胶原蛋白和SFS的Fn结合域中均​​存在九个残基。通过搜索俄克拉何马州 S。化脓基因数据库中，我们发现该基序也存在于 S的潜在细胞表面蛋白中。化脓。发现蛋白SFS以浓度依赖性方式抑制Fn和胶原之间的结合。

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