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首页> 外文期刊>Infection and immunity >Specific Antibody Responses to Three Schistosome-Related Carbohydrate Structures in Recently Exposed Immigrants and Established Residents in an Area of Schistosoma mansoni Endemicity
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Specific Antibody Responses to Three Schistosome-Related Carbohydrate Structures in Recently Exposed Immigrants and Established Residents in an Area of Schistosoma mansoni Endemicity

机译：曼氏血吸虫流行地区最近暴露的移民和既定居民对三种血吸虫相关碳水化合物结构的特异性抗体反应

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By the use of surface plasmon resonance spectroscopy, immunoglobulin G (IgG) subclass and IgM antibodies against three schistosome-derived carbohydrate structures, FLDN (Fucα1-3GalNAcβ1-4GlcNAcβ1-3Galα1), LDN-DF [GalNAcβ1-4(Fucα1-2Fucα1-3)GlcNAcβ1], and LDNF [GalNAcβ1-4(Fucα1-3)GlcNAcβ1-3Galα1], were measured in 184 previously unexposed Kenyan immigrants who moved into the Masongaleni area, where Schistosoma mansoni is endemic. They were sampled within their first year of exposure and again 2 years later. A cohort selected out of the original residents of the area, who had been exposed for many years, served as controls. Associations with responses to S. mansoni worm, egg (SEA), and cercarial (CERC) antigens were examined. In addition, we measured responses to keyhole limpet hemocyanin, a glycoprotein which carries glycan epitopes that are also expressed by schistosomes. Specific IgG1 responses were most pronounced against FLDN and LDN-DF and strongly associated with those previously measured to SEA and CERC. Similarly to previously published age profiles of IgG1 and IgG2 responses to SEA, levels of IgG1 against LDN-DF decreased with age. In contrast, specific IgM responses against the three schistosome-derived carbohydrate structures were most marked against LDNF. Our results indicate that, of the three glycan structures tested, the acute response against schistosome glycoconjugate antigens in young children is mainly directed against the LDN-DF epitope. The response to LDN-DF in older individuals and the responses to the two other epitopes were similar in the two cohorts, suggesting that these antigens are recognized in the early stages of infection and that the immune response persists. The biological significance of these observations needs further elucidation.
机译：通过使用表面等离振子共振光谱技术，针对三种血吸虫来源的碳水化合物结构，FLDN（Fucα1-3GalNAcβ1-4GlcNAcβ1-3Galα1），LDN-DF [GalNAcβ1-4（Fucα1-2Fucα1-3）的免疫球蛋白G（IgG）亚类和IgM抗体）GlcNAcβ1]和LDNF [GalNAcβ1-4（Fucα1-3）GlcNAcβ1-3Galα1]在184名先前未接触肯尼亚移民的人中进行了测量，这些移民进入了Masongaleni地区，那里的曼氏血吸虫是地方病。他们在接触的第一年和两年后再次取样。从该地区已暴露多年的原始居民中选出一个队列作为对照。与对 S的响应相关联。检查了曼氏蠕虫，卵（SEA）和子宫颈（CERC）抗原。此外，我们测量了对匙孔血蓝蛋白的反应，血红蛋白是一种糖蛋白，带有糖基表位，也由血吸虫表达。特异性IgG1应答对FLDN和LDN-DF最明显，并且与先前对SEA和CERC测得的应答强烈相关。与先前发表的针对SEA的IgG1和IgG2响应的年龄特征相似，针对LDN-DF的IgG1水平随年龄而降低。相反，针对三种血吸虫来源的碳水化合物结构的特异性IgM反应最明显地针对LDNF。我们的结果表明，在测试的三个聚糖结构中，幼儿对血吸虫糖共轭体抗原的急性反应主要针对LDN-DF表位。在这两个队列中，老年人对LDN-DF的反应和对其他两个表位的反应相似，这表明这些抗原在感染的早期就被识别，并且免疫反应持续存在。这些观察结果的生物学意义需要进一步阐明。

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