首页> 外国专利> DNA STRUCTURES CONTAINING NUCLEIC ACIDS ENCODING HUMAN INFLUENZAL VIRUSES, POLYNUCLEOTIDE VACCINE CONTAINING DNA STRUCTURES INDUCING THE FORMATION OF NEUTRALIZING ANTIBODIES AND SPECIFIC CYTOSTATIC T-LYMPHOCYTES, EXPRESSION VECTORS, PROTECTION METHOD FROM INFLUENZAL VIRUS INFECTION AND THE USE OF THE VIRUSES AND GENES THEREOF FOR INDUCING IN VIVO IMMUNE RESPONSE

DNA STRUCTURES CONTAINING NUCLEIC ACIDS ENCODING HUMAN INFLUENZAL VIRUSES, POLYNUCLEOTIDE VACCINE CONTAINING DNA STRUCTURES INDUCING THE FORMATION OF NEUTRALIZING ANTIBODIES AND SPECIFIC CYTOSTATIC T-LYMPHOCYTES, EXPRESSION VECTORS, PROTECTION METHOD FROM INFLUENZAL VIRUS INFECTION AND THE USE OF THE VIRUSES AND GENES THEREOF FOR INDUCING IN VIVO IMMUNE RESPONSE

机译：包含编码人类流感病毒的核酸的DNA结构，包含核酸结构的多核苷酸疫苗，诱导中和抗体和特定的细胞周期T淋巴细胞的中性抗原和表达的病毒，细菌的表达免疫反应

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摘要

In the present invention there is disclosed a DNA construct comprising nucleic acid encoding an influenza virus gene, wherein the influenza virus gene encodes nucleoprotein, hemagglutinin, polymerase, matrix, or non-structural human influenza virus gene products, wherein said DNA construct is capable of inducing the expression of an antigenic influenza virus gene product which induces an influenza virus specific immune response upon introduction of said DNA construct into animal tissues in vivo and resultant uptake of the DNA construct by cells which express the encoded influenza gene. The above specified DNA construct consists of (a) a region of the CMVIntA promoter and a BGH region for terminating transcription from SEQ.ID:11, b) prokaryotic start of replication, c) gene for resistance to antibiotics and d)open reading frame disposed between the promoter and transcription terminating signal from the component under a) and controlling gene expression of the influenza virus. In the present invention there is also claimed a polynucleotide vaccine containing these constructs as well as use of constructs for preparing said vaccine.

机译：在本发明中，公开了一种DNA构建体，其包含编码流感病毒基因的核酸，其中所述流感病毒基因编码核蛋白，血凝素，聚合酶，基质或非结构性人流感病毒基因产物，其中所述DNA构建体能够诱导抗原性流感病毒基因产物的表达，所述抗原性流感病毒基因产物在体内将所述DNA构建体引入动物组织后诱导流感病毒特异性免疫反应，并导致表达编码流感基因的细胞对DNA构建体的吸收。上述指定的DNA构建体由（a）CMVIntA启动子区域和用于终止SEQ.ID:11转录的BGH区域组成，b）原核复制开始，c）抗生素抗性基因和d）开放阅读框在a）下，其位于启动子和转录终止信号之间，并控制流感病毒的基因表达。在本发明中，还要求保护一种含有这些构建体的多核苷酸疫苗，以及该构建体在制备所述疫苗中的用途。

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公开/公告号CZ9502373A3

专利类型
公开/公告日1996-02-14

原文格式PDF
申请/专利权人 MERCK AND CO. INC;VICAL INCORPORATED;
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申请/专利号CZ19950002373
发明设计人 MONTGOMERY DONNA L.;ULMER JEFFREY B.;DONNELLY JOHN J.;LIU MARGARET A.;SHIVER JOHN W.;DWARKI VARAVANI J.;PARKER SUEZANNE E.;
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申请日1994-03-14
分类号C12N15/44;A61K31/70;A61K48/00;
国家 CZ
入库时间 2022-08-22 03:54:35

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