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首页> 外文期刊>Infection and immunity >High Expression of a C Protein β Antigen Gene among Invasive Strains from Certain Clonally Related Groups of Type Ia and Ib Group B Streptococci
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High Expression of a C Protein β Antigen Gene among Invasive Strains from Certain Clonally Related Groups of Type Ia and Ib Group B Streptococci

机译：C蛋白β抗原基因在Ia型和Ib型B族链球菌的某些克隆相关群体中的侵袭性菌株中的高表达

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Serotyped strains of group B streptococci can be divided into subtypes based on restriction endonuclease digestion patterns (RDP). Profiles of cell-bound proteins were compared among RDP types. Proteins that showed a remarkable difference in the degree of expression were found among strains of RDP Ia-3, which has been considered potentially virulent, as well as of RDP Ib-1. For RDP Ia-3 strains, the protein was predominant in strains from cerebrospinal fluid (CSF) but was mostly a minor component in other strains. For RDP Ib-1 strains, the protein was predominant in strains from CSF, showed diversity in strains from blood, and was mostly a minor component in other strains. By N-terminal sequencing analysis, the protein was identified as a C protein β antigen. The level of bound immunoglobulin A (IgA) or anti-β antigen monoclonal antibody correlated with the level of expressed β antigen, and invasive strains showed remarkably high levels of binding; the exception was a CSF-derived strain of RDP Ib-1 which produced a large amount of β antigen and showed a high level of binding of anti-β antigen monoclonal antibody but no IgA binding. PCR-based amplification revealed that the β antigen gene was detected in all RDP Ia-3 and Ib-1 strains but was not found in any strains of other RDP types. Competitive reverse transcriptase PCR demonstrated that the difference in the amount of protein produced was due to the difference in the level of expression of the β antigen mRNA. Our findings imply that differences in gene expression for a protein may contribute to the invasiveness of RDP Ia-3 and Ib-1 strains for the host.
机译：B型链球菌的血清型菌株可根据限制性核酸内切酶消化模式（RDP）分为亚型。在RDP类型之间比较了细胞结合蛋白的概况。在RDP Ia-3和RDP Ib-1的菌株之间发现了表达程度差异显着的蛋白质。对于RDP Ia-3菌株，该蛋白在脑脊液（CSF）的菌株中占主导地位，但在其他菌株中则主要是次要成分。对于RDP Ib-1菌株，该蛋白在CSF菌株中占主导地位，在血液中显示出多样性，在其他菌株中大多是次要成分。通过N末端测序分析，该蛋白被鉴定为C蛋白β抗原。结合的免疫球蛋白A（IgA）或抗β抗原单克隆抗体的水平与表达的β抗原的水平相关，侵袭性菌株显示出很高的结合水平。例外是CSF衍生的RDP Ib-1菌株产生大量β抗原，并显示出高水平的抗β抗原单克隆抗体结合力，但没有IgA结合力。基于PCR的扩增显示，在所有RDP Ia-3和Ib-1菌株中均检测到β抗原基因，但在其他RDP类型的任何菌株中均未发现。竞争性逆转录酶PCR证明蛋白质产生量的差异是由于β抗原mRNA表达水平的差异所致。我们的发现暗示蛋白质基因表达的差异可能有助于RDP Ia-3和Ib-1菌株对宿主的侵袭性。

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