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首页> 外文期刊>Infection and immunity >Antibody Avidity and Immunoglobulin G Isotype Distribution following Immunization with a Monovalent Meningococcal B Outer Membrane Vesicle Vaccine
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Antibody Avidity and Immunoglobulin G Isotype Distribution following Immunization with a Monovalent Meningococcal B Outer Membrane Vesicle Vaccine

机译：单价脑膜炎球菌B外膜囊泡疫苗免疫后的抗体亲和力和免疫球蛋白G型分布

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The avidity maturation and immunoglobulin G (IgG) isotype distribution of antibodies after vaccination with a meningococcal B outer membrane vesicle (OMV) vaccine were evaluated as indicators of protective immunity. Pre- and postvaccination sera from 134 healthy toddlers (ages, 2 to 3 years) immunized with a monovalent meningococcal B OMV (serosubtype P1.7-2,4) vaccine adsorbed with AlPO4 or Al(OH)3 were analyzed by enzyme-linked immunosorbent assay (ELISA) methods. The children were vaccinated three times with intervals of 3 to 6 weeks between vaccinations or twice with an interval of 6 to 10 weeks between vaccinations. A booster was given after 20 to 40 weeks. The avidity index (AI) of antibodies increased significantly during the primary series of vaccinations and after the booster was given. No differences in AIs were found when the results obtained with the two vaccination schedules or with the two adjuvants were compared. After vaccination, IgG1 was the predominant IgG isotype, followed by IgG3. No IgG2 or IgG4 was detected. There was a strong correlation between serum bactericidal activity (SBA) and ELISA titers (r = 0.85 [P < 0.0001] for total IgG, r = 0.83 for IgG1 [P < 0.0001], r = 0.82 for IgG3 [P < 0.0001], and r = 0.84 [P < 0.0001] for the avidity titer). When two subgroups with similar anti-OMV IgG levels were compared before and after the booster vaccination, the higher AI after the booster vaccination was associated with significantly increased SBA. We concluded that avidity maturation occurs after vaccination with a monovalent meningococcal B OMV vaccine, especially after boosting, as indicated by a significant increase in the AI. Vaccination with the monovalent OMV vaccine induced mainly IgG1 and IgG3 isotypes, which are considered to be most important for protection against meningococcal disease. An increase in the AI of antibodies is associated with increased SBA, independent of the level of specific IgG and the IgG isotype distribution. Measuring the AI and IgG isotype distribution of antibodies after vaccination can be a supplementary method for predicting protective immunity for evaluation in future phase III trials with meningococcal serogroup B vaccines.
机译：将脑膜炎球菌B外膜囊泡（OMV）疫苗接种后的抗体的亲和力成熟度和免疫球蛋白G（IgG）同种型分布评估为保护性免疫的指标。用吸附有AlPO 4 或Al（OH）的单价脑膜炎球菌B OMV（血清亚型P1.7-2,4）疫苗免疫的134名健康学龄前儿童（2至3岁）的免疫前后酶联免疫吸附法（ELISA）分析 3 。对儿童进行了3次疫苗接种，两次免疫间隔3至6周，两次两次疫苗间隔6至10周。 20至40周后加强免疫。在最初的一系列疫苗接种期间和加强接种后，抗体的亲和力指数（AI）显着提高。比较两种疫苗接种方案或两种佐剂获得的结果时，没有发现AI差异。接种疫苗后，IgG1是主要的IgG同种型，其次是IgG3。未检测到IgG2或IgG4。血清杀菌活性（SBA）与ELISA滴度之间有很强的相关性（ r = 0.85 [ P <0.0001]对于总IgG， r 对于IgG1 [ P <0.0001] = 0.83，对于 r = 0.82对于IgG3 [ P <0.0001]和 r = 0.84 [ P <0.0001]（亲和力滴度）。当在加强疫苗接种前后比较具有相似抗-OMV IgG水平的两个亚组时，加强疫苗接种后较高的AI与SBA显着增加有关。我们得出的结论是，用单价脑膜炎球菌B OMV疫苗接种后，尤其是加强免疫后，亲合力就会发生，这是AI的显着增加所表明的。单价OMV疫苗的疫苗接种主要诱导IgG1和IgG3同种型，这被认为对预防脑膜炎球菌疾病最重要。抗体AI的增加与SBA的增加有关，而与特异性IgG的水平和IgG同种型分布无关。测量疫苗接种后抗体的AI和IgG同种型分布可以是预测保护性免疫力的补充方法，以便在将来的脑膜炎球菌B群血清疫苗的III期试验中进行评估。

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