首页> 外国专利> Process for high-level expression of the porin group B meningococcal protein or its outer membrane fusion protein in E coli process for the purification of the group B meningococcal porin protein or its external membrane fusion protein meningococcal group B or its fusion protein on the outer membrane porin group B meningococcal protein or its fusion protein on the outer membrane substantially pure vaccine containing

Process for high-level expression of the porin group B meningococcal protein or its outer membrane fusion protein in E coli process for the purification of the group B meningococcal porin protein or its external membrane fusion protein meningococcal group B or its fusion protein on the outer membrane porin group B meningococcal protein or its fusion protein on the outer membrane substantially pure vaccine containing

机译:在大肠杆菌中高表达B蛋白组B脑膜炎球菌或其外膜融合蛋白的方法在B膜上纯化B组脑膜炎球菌蛋白或其外膜融合蛋白的方法B外膜融合蛋白在外膜上的孔蛋白B组脑膜炎球菌蛋白质或其融合蛋白含有

摘要

The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins yard fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.
机译:总的来说,本发明涉及高表达外膜蛋白B脑膜炎球菌B组孔蛋白及其融合蛋白的方法。特别地,本发明涉及在大肠杆菌中表达外膜蛋白B脑膜炎球菌组孔蛋白的方法,其中所述脑膜炎球菌B组孔蛋白蛋白质的融合蛋白占在大肠杆菌中表达的总蛋白的2%以上。 。本发明还涉及纯化和重折叠B脑膜炎球菌B蛋白蛋白及其融合蛋白的方法,以及它们在疫苗中的用途。

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