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Neisseria meningitidis, high-level expression, purification and refolding of group B outer membrane porin proteins

机译:脑膜炎奈瑟氏球菌,B组外膜孔蛋白的高水平表达,纯化和重折叠

摘要

The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins yard fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.
机译:总的来说,本发明涉及高表达外膜蛋白B脑膜炎球菌B组孔蛋白及其融合蛋白的方法。特别地,本发明涉及在大肠杆菌中表达外膜蛋白B脑膜炎球菌组孔蛋白的方法,其中所述脑膜炎球菌B组孔蛋白蛋白质的融合蛋白占在大肠杆菌中表达的总蛋白的2%以上。 。本发明还涉及纯化和重折叠B脑膜炎球菌B蛋白蛋白及其融合蛋白的方法,以及它们在疫苗中的用途。

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