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首页> 外文会议>American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics >Protein Composition of a Vesicular Vaccine Carrier Derived from the Outer Membrane of Neisseria meningitidis
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Protein Composition of a Vesicular Vaccine Carrier Derived from the Outer Membrane of Neisseria meningitidis

机译：衍生自Neisseria Meningitidis的外膜的蛋白质组成衍生的血管疫苗载体

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The complete proteome and the surface proteome of OMPC have been determined by tandem mass spectrometry. A total of 540 proteins have been identified with a predicted false positive rate of 0.5percent. OMPC obtained from a serogroup B strain (B11) is more readily characterized when compared to a serogroup C database (FAM18). ?The 10 most abundant OMPC proteins account for 75percent of the total protein signal intensity. PorA and PorB are the most abundant proteins in OMPC and account for approximately 50percent of the total protein signal intensity. Por A and Por B are only poorly accessible on the surface. The most surface accessible proteins of OMPC are rmpM and NadA. 344 proteins were found to have reacted with N-acetylhomocysteine thiolactone. Chemically reacted proteins account for >95percent of the total protein signal intensity. 36 predicted outer membrane proteins account for 75percent of the total protein signal intensity. OMPC Proteins that are not membrane associated appear to be nonspecifically attached to the OMPC surface. No evidence has been found for significant amounts of proteins that are trapped inside the OMPC. Chemical reaction efficiency of PorB Lysine residues are not driven by surface accessibility and can be only empirically determined. Factors such as, internal salt bridges, shielding by the membrane and shielding by oligomerization and protein complex formation seem to play an important role.
机译：通过串联质谱法确定了OMPC的完整蛋白质组和表面蛋白质组。已经鉴定了总共540个蛋白质，预测的假阳性率为0.5％。与Serogroup C数据库（FAM18）相比，从血清群B菌株（B11）获得的OMPC更容易表征。 ？10个最丰富的OMPC蛋白占总蛋白质信号强度的75％。 Pora和Porb是OMC中最丰富的蛋白质，并且占总蛋白质信号强度的大约50％。 POR A和POR B才能在表面上可达。 OMPC的最表面可接近的蛋白质是RMPM和NADA。发现344个蛋白质与N-乙酰卤代细胞硫内酯反应。化学反应的蛋白质占>总蛋白质信号强度的95％。 36预测的外膜蛋白占总蛋白质信号强度的75％。不相关的OMPC蛋白质似乎是非特异性地连接到OMPC表面。没有发现捕获在OMPC内部的大量蛋白质的证据。 Porb赖氨酸残基的化学反应效率不是通过表面可访问性驱动，并且可以仅经过经验确定。诸如内盐桥，膜屏蔽的因素，通过寡聚化和蛋白质复杂形成屏蔽似乎发挥着重要作用。

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