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首页> 外文期刊>Microbiology >A linear B-cell epitope on the class 3 outer-membrane protein of Neisseria meningitidis recognized after vaccination with the Norwegian group B outer-membrane vesicle vaccine
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A linear B-cell epitope on the class 3 outer-membrane protein of Neisseria meningitidis recognized after vaccination with the Norwegian group B outer-membrane vesicle vaccine

机译：与挪威群B外膜囊泡疫苗接种疫苗接种后，培条脑膜脑膜三种外膜蛋白的线性B细胞表位

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Summary: The class 3 outer-membrane protein (OMP) of Neisseria meningitidis is a potential target for bactericidal and opsonic antibodies in humans. Synthetic peptides spanning the class 3 OMP from the vaccine strain 44/76 (B: 15:P1.7,16:L3,7) were synthesized on pins and screened with serum obtained from Norwegian adolescents immunized with a meningococcal serogroup B outer-membrane vesicle (OMV) vaccine. A strong IgG response to a single peptide (19FHQNGQVTEVTT30) located within loop 1 (VR1) was stimulated after three doses of OMV vaccine in three vaccinees selected on the basis of their antibody response to class 3 OMP. No clear linear B-cell epitopes were recognized by four different murine serotype 15-specific mAbs. A 23mer peptide (D63b2) containing loop 1 of the class 3 OMP was synthesized, and the IgG responses were measured in pre- and post-vaccination serum from 27 vaccinees. Specific IgG rose significantly in 37% of vaccinees 6 weeks after the second dose and in 74% of the vaccinees 6 weeks after the third dose of the OMV vaccine. Most immune sera reacted distinctly on immunoblots with denatured class 3 OMP, and the immunoblotting reactivity correlated strongly with concentration of the IgG antibodies specific for peptide D63b2. When added to a post-vaccination serum from one vaccinee, peptide D63b2 competed efficiently with the class 3 OMP for specific antibody binding on immunoblots and in pin ELISA. The results show that the significant part of the humoral response to the meningococcal class 3 OMP elicited by vaccination with the Norwegian OMV vaccine was directed against a single continuous epitope.
机译：发明内容：Neisseria Meningitidis的3类外膜蛋白（OMP）是人类杀菌和Opsonic抗体的潜在目标。在引脚上合成了从疫苗菌株44/76（B：15：15：p1.7,16：L3,7）的合成肽在销中合成并筛选从用脑膜炎球菌血清血清B外膜免疫的挪威青少年获得的血清囊泡（OMV）疫苗。在基于第3类OMP的抗体反应的三次疫苗中，在三次疫苗中刺激对环1（VR1）内的单个肽（VR1）内的单个肽（19FHQNGQVTEVTT30）的强烈IgG响应。不透明的线性B细胞表位被四种不同的鼠血清型15特异性mAb识别。合成了含有3类OMP的环1的23mer肽（D63B2），并在来自27个疫苗的疫苗接种前和接种后疫苗中测量IgG响应。特定IgG在第二剂后6周后显着增加37％的疫苗，并在OMV疫苗的第三剂量后6周内疫苗的74％。大多数免疫血清在具有变性的3级OMP的免疫印迹中明显地反应，并且免疫印迹反应性与肽D63B2特异的IgG抗体的浓度强烈相关。当从一个疫苗中添加到接种后疫苗血清时，肽D63b2有效地竞争3阶段，在免疫印迹和销ELISA上具有3种特异性抗体结合。结果表明，通过与挪威OMV疫苗接种疫苗引起的脑膜炎球菌类3的体液反应的重要部分是针对单一连续表位的。

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