...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Intracellular Adhesion Molecule 1 Plays a Key Role in Acquired Immunity to Salmonellosis
【24h】

Intracellular Adhesion Molecule 1 Plays a Key Role in Acquired Immunity to Salmonellosis

机译：细胞内粘附分子1在沙门氏菌病的获得性免疫中起关键作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study investigated the role of intracellular adhesion molecule 1 (ICAM-1) during Salmonella enterica serovar Typhimurium infection of mice. We show that ICAM-1 is expressed in and around granulomas on day 4 of infection in wild-type mice. However, when naive ICAM-1?/? mice were challenged with a sublethal dose of serovar Typhimurium, there were no detectable differences in systemic bacterial burden over the first 9 days of infection compared to wild-type control mice. When mice were immunized with the S. enterica serovar Typhimurium vaccine strain SL2361 and then challenged with the virulent S. enterica serovar Typhimurium strain C5, 100% of the ICAM-1?/? mice succumbed to infection, compared to 30% of wild-type mice. T-cell responses, as measured by activation via interleukin-2 production, as well as antibody responses were comparable in the ICAM-1?/? and wild-type mice. Following challenge, counts in organs were significantly higher in the ICAM-1?/? mice, and histological examination of organs showed pathological differences. Strain SL3261-immunized wild-type mice had cellular infiltrate and normal granuloma formation in the liver and spleen on days 5 and 10 after challenge with strain C5. ICAM-1?/? mice had a similar infiltrate on day 5, whereas on day 10 the infiltrate was more widespread and there were fewer macrophages associated with the granulomas. High circulating levels of tumor necrosis factor alpha and gamma interferon, as well as a high burden of strain C5 in the blood, accompanied the differences in histopathology. In this study we show that ICAM-1 plays a critical role during rechallenge of immunized mice with virulent S. enterica serovar Typhimurium.
机译：这项研究调查了细胞内粘附分子1（ICAM-1）在小鼠沙门氏菌血清鼠伤寒感染中的作用。我们显示ICAM-1在野生型小鼠感染的第4天在肉芽肿中及其周围表达。但是，当用低于致死剂量的鼠伤寒鼠伤寒血清攻击幼稚ICAM-1 ？/？小鼠时，与野生型对照相比，在感染的前9天中，系统细菌负荷没有可检测到的差异老鼠。当用 S免疫小鼠时。肠炎血清型鼠伤寒疫苗菌株SL2361，然后用有毒的 S攻击。肠型鼠伤寒沙门氏菌C5株，ICAM-1 ？/？小鼠死于感染的比例为100％，而野生型小鼠为30％。通过ICAM-1 ？/？和野生型小鼠的T细胞反应（通过白介素2产生的激活来测量）以及抗体反应是可比的。攻击后，ICAM-1 ？/？小鼠的器官计数显着更高，器官的组织学检查显示病理学差异。用菌株C3261免疫的野生型小鼠在用菌株C5攻击后第5天和第10天在肝和脾中具有细胞浸润和正常肉芽肿形成。 ICAM-1 ？/？小鼠在第5天有类似的浸润，而在第10天，浸润更广泛，与肉芽肿相关的巨噬细胞更少。肿瘤坏死因子α和γ干扰素的高循环水平以及血液中C5株的高负担伴随着组织病理学的差异。在这项研究中，我们表明ICAM-1在强毒 S免疫小鼠的再攻击过程中起着关键作用。肠炎血清型鼠伤寒。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号