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首页> 外文期刊>Infection and immunity >PspA Protects Streptococcus pneumoniae from Killing by Apolactoferrin, and Antibody to PspA Enhances Killing of Pneumococci by Apolactoferrin
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PspA Protects Streptococcus pneumoniae from Killing by Apolactoferrin, and Antibody to PspA Enhances Killing of Pneumococci by Apolactoferrin

机译：PspA保护肺炎链球菌免受Apolactoferrin的杀伤，PspA抗体增强Apolactoferrin对肺炎球菌的杀灭作用。

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Lactoferrin is an important component of innate immunity through its sequestration of iron, bactericidal activity, and immune modulatory activity. Apolactoferrin (ALF) is the iron-depleted form of lactoferrin and is bactericidal against pneumococci and several other species of bacteria. We observed that lactoferricin (LFN), an 11-amino-acid peptide from the N terminus of lactoferrin, is bactericidal for Streptococcus pneumoniae. Strains of S. pneumoniae varied in their susceptibility to ALF. Lactoferrin is bound to the pneumococcal surface by pneumococcal surface protein A (PspA). Using mutant PspA? pneumococci of four different strains, we observed that PspA offers significant protection against killing by ALF. Knockout mutations in genes for two other choline-binding proteins (PspC and PcpA) did not affect killing by ALF. PspA did not have to be attached to the bacterial surface to inhibit killing, because the soluble recombinant N-terminal half of PspA could prevent killing by both ALF and LFN. An 11-amino-acid fragment of PspA was also able to reduce the killing by LFN. Antibody to PspA enhanced killing by lactoferrin. These findings suggested that the binding of ALF to PspA probably blocks the active site(s) of ALF that is responsible for killing.
机译：乳铁蛋白通过隔离铁，杀菌活性和免疫调节活性，是先天免疫的重要组成部分。 Apolactoferrin（ALF）是一种贫铁形式的乳铁蛋白，对肺炎球菌和其他几种细菌具有杀菌作用。我们观察到，乳铁蛋白（LFN）是乳铁蛋白N末端的11个氨基酸的肽，对肺炎链球菌具有杀菌作用。 S株。肺炎对ALF的敏感性不同。乳铁蛋白通过肺炎球菌表面蛋白A（PspA）与肺炎球菌表面结合。使用四种不同菌株的突变型PspA ？肺炎链球菌，我们观察到PspA对ALF杀伤具有明显的保护作用。其他两种胆碱结合蛋白（PspC和PcpA）的基因敲除突变不会影响ALF的杀伤力。 PspA不必附着在细菌表面上以抑制杀伤，因为PspA的可溶性重组N末端一半可以防止ALF和LFN杀伤。 PspA的11个氨基酸片段也能够减少LFN的杀伤作用。 PspA抗体增强了乳铁蛋白的杀伤力。这些发现表明，ALF与PspA的结合可能阻断了负责杀死ALF的活性位点。

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