• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Bioluminescence Imaging of Toxoplasma gondii Infection in Living Mice Reveals Dramatic Differences between Strains
【24h】

Bioluminescence Imaging of Toxoplasma gondii Infection in Living Mice Reveals Dramatic Differences between Strains

机译：弓形虫感染在活体小鼠中的生物发光成像揭示了菌株之间的显着差异。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We examined the in vivo growth, dissemination, and reactivation of strains of the protozoan parasite Toxoplasma gondii using a bioluminescence-based imaging system. Two T. gondii strains, one with a highly virulent disease phenotype in mice (S23) and the other with a 1,000-fold-lower virulence phenotype (S22), were engineered to stably express the light-emitting protein luciferase. One clone of each wild-type strain was isolated, and the two clones (S23-luc7 and S22-luc2) were found to express similar levels of luciferase. Mice were infected intraperitoneally with S23-luc7 (50 or 5 parasites) or S22-luc2 (500, 50, or 5 parasites), and the progress of the infections was examined noninvasively following injection of the substrate for luciferase, d-luciferin. In mice infected with 50 S23-luc7 parasites, the parasites grew exponentially within the peritoneal cavity (as measured by light emitted from luciferase-expressing parasites) during days 1 to 10 p.i., and this proliferation continued until there was severe disease. In mice infected with 500 S22-luc2 parasites, the parasites proliferated in a fashion similar to the S23-luc7 proliferation during days 1 to 6, but this was followed by a precipitous drop in the signal to levels below the limit of detection. Using this technique, we were also able to observe the process of reactivation of T. gondii in chronically infected mice. After treatment with dexamethasone, we detected reactivation of toxoplasmosis in mice infected with S23-luc7 and S22-luc2. During reactivation, growth of S23-luc7 was initially detected primarily in the head and neck area, while in S22-luc2-infected mice the parasites were detected primarily in the abdomen. This method has great potential for identifying important differences in the dissemination and growth of different T. gondii strains, especially strains with dramatically different disease outcomes.
机译：我们使用基于生物发光的成像系统检查了原生动物寄生虫<弓形虫>弓形虫的体内生长，传播和再活化。两个 T。对刚地菌株进行了改造，使其稳定表达发光蛋白荧光素酶，其中一种在小鼠中具有高毒力疾病表型（S23），而另一种具有低1000倍的毒力表型（S22）。分离出每个野生型菌株的一个克隆，发现两个克隆（S23-luc7和S22-luc2）表达相似水平的荧光素酶。用S23-luc7（50或5个寄生虫）或S22-luc2（500、50或5个寄生虫）腹膜内感染小鼠，并在注射荧光素酶d-荧光素底物后无创检查感染的进程。在感染了50个S23-luc7寄生虫的小鼠中，该寄生虫在腹膜腔内呈指数增长（通过表达萤光素酶的寄生虫发出的光测量）在p.i.的第1天至第10天，这种增殖一直持续到出现严重疾病为止。在感染了500个S22-luc2寄生虫的小鼠中，这些寄生虫在第1至6天以类似于S23-luc7增殖的方式增殖，但是随后信号急剧下降至低于检测极限的水平。使用此技术，我们还能够观察到 T的重新激活过程。弓形虫在慢性感染的小鼠中。用地塞米松治疗后，我们在感染了S23-luc7和S22-luc2的小鼠中检测到弓形虫病的再激活。在重新激活过程中，最初主要在头部和颈部检测到S23-luc7的生长，而在S22-luc2感染的小鼠中，主要在腹部检测到了寄生虫。该方法具有巨大潜力，可确定不同T的传播和生长中的重要差异。弓形虫菌株，尤其是疾病结果截然不同的菌株。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号