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Infection of Newborn Piglets with Bordetella pertussis: a New Model for Pertussis

机译：百日咳博德特氏菌感染新生仔猪：百日咳的新模型

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Bordetella pertussis is the causative agent of pertussis or whooping cough. This bacterium is a human pathogen that under experimental conditions also infects selected rodents and primates. Here, we show for the first time that newborn piglets can be infected with B. pertussis when it is delivered intrapulmonarily. Infected piglets displayed fever and respiratory symptoms, such as nasal discharge, nonparoxysmal coughing, and breathing difficulties. Eventually, all infected animals developed severe bronchopneumonia, which in some cases was combined with a fibrinous pleuritits. Immunohistochemical staining revealed the presence of large numbers of B. pertussis cells within airways, adhering to the epithelial lining or phagocytosed by macrophages and neutrophils. Viable bacteria were reisolated from bronchoalveolar lavages and lung lesions for more than 10 days postinfection. The systemic presence of pertussis toxin was shown by hypoglycemia, lymphocytosis, and induction of a clustered pattern of CHO cells by serum and bronchoalveolar lavage samples. Thus, a large-animal model for pertussis was developed, which should complement existing rodent models for identifying the immune responses relevant to the design of new vaccines. In particular, this model should help researchers analyze the roles of both maternal and mucosal immunity in disease protection against pertussis and should ultimately assist in the design of new vaccines for early life protection.
机译：百日咳博德特氏菌是百日咳或百日咳的病原。该细菌是人类病原体，在实验条件下也会感染选定的啮齿动物和灵长类动物。在这里，我们首次展示了新生仔猪可以被 B感染。百日咳通过肺内输送。受感染的仔猪表现出发烧和呼吸道症状，例如鼻涕，非阵发性咳嗽和呼吸困难。最终，所有被感染的动物都出现了严重的支气管肺炎，在某些情况下还伴有纤维化胸膜炎。免疫组织化学染色显示大量的 B。气道内的百日咳细胞粘附在上皮衬里或被巨噬细胞和嗜中性粒细胞吞噬。感染后10天内，从支气管肺泡灌洗液和肺部病变中分离出活菌。低血糖，淋巴细胞增多以及血清和支气管肺泡灌洗液样本诱导的CHO细胞簇状显示出百日咳毒素的全身存在。因此，开发了百日咳大动物模型，该模型应补充现有的啮齿动物模型以鉴定与新疫苗设计相关的免疫应答。特别是，该模型应有助于研究人员分析母体和粘膜免疫在百日咳疾病保护中的作用，并最终协助设计用于早期生命保护的新疫苗。

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