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首页> 外文期刊>Infection and immunity >Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva
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Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in Saliva

机译：放线杆菌聚合体与唾液中的核梭形杆菌和韦永氏菌种建立了相互的生物膜群落

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Human oral bacterial pathogens grow in attached multispecies biofilm communities. Unattached cells are quickly removed by swallowing. Therefore, surface attachment is essential for growth, and we investigated multispecies community interactions resulting in mutualistic growth on saliva as the sole nutritional source. We used two model systems, saliva-coated transferable solid-phase polystyrene pegs (peg biofilms) and flow cells with saliva-coated glass surfaces. Fluorescent antibody staining and image analysis were used to quantify the biomass in flow cells, and quantitative real-time PCR with species-specific primers was used to quantify the biomass in peg biofilms. Veillonella sp. strain PK1910, Aggregatibacter actinomycetemcomitans JP2, and Fusobacterium nucleatum ATCC 10953 were unable to grow as single species in flow cells. Only A. actinomycetemcomitans grew after 36 h when peg biofilms remained submerged in saliva from the time of inoculation. Mixed-species coaggregates were used for two- and three-species inoculation. The biomass in two-species biofilms increased in both systems when Veillonella sp. strain PK1910 was present as one of the partners. Enhanced growth of all strains was observed in three-species biofilms in flow cells. Interestingly, in flow cells F. nucleatum and A. actinomycetemcomitans exhibited mutualism, and, although F. nucleatum was unable to grow with either of the other species in the peg system, F. nucleatum stimulated the growth of Veillonella sp. and together these two organisms increased the total biomass of A. actinomycetemcomitans in three-species peg biofilms. We propose that mutualistic two-species and multispecies oral biofilm communities form in vivo and that mutualism between commensal veillonellae and late colonizing pathogens, such as aggregatibacteria, contributes to the development of periodontal disease.
机译：人类口腔细菌病原体生长在附着的多种生物膜群落中。吞咽可迅速去除未附着的细胞。因此，表面附着对于生长是必不可少的，我们调查了多种物种的相互作用，从而导致唾液作为唯一营养来源的相互生长。我们使用了两种模型系统，唾液涂层的可转移固相聚苯乙烯钉（peg生物膜）和带有唾液涂层的玻璃表面的流通池。使用荧光抗体染色和图像分析来定量流动池中的生物量，并使用具有物种特异性引物的定量实时PCR来定量钉住生物膜中的生物量。 Veillonella sp。 PK1910菌株，集热放线杆菌 JP2和核融合杆菌 ATCC 10953不能在流通池中作为单个物种生长。仅 A。接种后36小时后，唾液中仍挂有钉子生物膜，从而放线菌放牧。混合物种聚集体用于两种和三种物种的接种。当 Veillonella sp。时，两种系统中两种生物膜中的生物量均增加。 PK1910菌株是伴侣之一。在流通池中的三种生物膜中观察到所有菌株的生长均增强。有趣的是，在流通池中。核和 A。放线菌（Cominomycetemcomitans）表现出共生关系，尽管是 F。核不能与钉系统 F中的任何其他物种一起生长。核刺激了 Veillonella sp。的生长。这两种生物共同增加了 A的总生物量。三种物种钉生物膜中的放线菌我们建议在体内形成互惠的两物种和多物种口腔生物膜群落，共生的veillonellae和晚期定植病原体（例如聚合细菌）之间的互惠关系有助于牙周疾病的发展。

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