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首页> 外文期刊>Infection and immunity >Developmental Expression of a Trypanosoma cruzi Phosphoinositide-Specific Phospholipase C in Amastigotes and Stimulation of Host Phosphoinositide Hydrolysis
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Developmental Expression of a Trypanosoma cruzi Phosphoinositide-Specific Phospholipase C in Amastigotes and Stimulation of Host Phosphoinositide Hydrolysis

机译：锥虫锥虫磷酸肌醇特异性磷脂酶C的发育表达和宿主磷酸肌醇水解的刺激。

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摘要

Phosphoinositide phospholipase C (PI-PLC) plays an essential role in cell signaling. A unique Trypanosoma cruzi PI-PLC (TcPI-PLC) is lipid modified in its N terminus and localizes to the outer surface of the plasma membrane of amastigotes. We show here that TcPI-PLC is developmentally regulated in amastigotes and shows two peaks of surface expression during the developmental cycle of T. cruzi, the first immediately after differentiation of trypomastigotes into amastigotes and the second before differentiation of amastigotes into trypomastigotes. Surface expression of TcPI-PLC coincides with phosphatidylinositol 4,5-bisphosphate (PIP2) depletion in the host cell membrane and with an increase in the levels of its product, inositol 1,4,5-trisphosphate. During extracellular differentiation, PI-PLC is secreted into the incubation medium. Maximal early expression of TcPI-PLC on the surface of amastigotes and PIP2 depletion coincide with host cytoskeletal changes, Ca2+ signaling, and transcriptional responses described previously. The presence of TcPI-PLC on the outer surface of the plasma membrane of the parasite and the capacity to be secreted and to alter host phospholipids are novel mechanisms of the host-parasite interaction.
机译：磷酸肌醇磷脂酶C（PI-PLC）在细胞信号传导中起着至关重要的作用。独特的 Trypanosoma cruzi PI-PLC（ Tc PI-PLC）在其N端进行了脂质修饰，并位于变形虫质膜的外表面。我们在这里显示 Tc PI-PLC在变形虫中受到发育调控，并且在 T的发育周期中显示两个表面表达峰。 cruzi ，第一个是将锥体鞭毛虫分化为变形虫后，第二个是将鞭毛鞭毛虫分化为锥虫。 Tc PI-PLC的表面表达与宿主细胞膜中的磷脂酰肌醇4,5-双磷酸（PIP 2 ）消耗相符，并且其产物水平增加，肌醇1,4,5-三磷酸。在细胞外分化过程中，PI-PLC被分泌到孵育培养基中。 Tc PI-PLC在变形虫表面和PIP 2 耗竭的最大早期表达与宿主细胞骨架变化，Ca 2 + 信号传导和转录反应如前所述。寄生虫的质膜外表面上存在 Tc PI-PLC以及分泌和改变宿主磷脂的能力是宿主-寄生虫相互作用的新机制。

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