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首页> 外文期刊>Infection and immunity >Examination of Type IV Pilus Expression and Pilus-Associated Phenotypes in Kingella kingae Clinical Isolates
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Examination of Type IV Pilus Expression and Pilus-Associated Phenotypes in Kingella kingae Clinical Isolates

机译：国王金氏菌临床分离株中IV型毛发表达和毛发相关表型的检查

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Kingella kingae is a gram-negative bacterium that is being recognized increasingly as a cause of septic arthritis and osteomyelitis in young children. Previous work established that K. kingae expresses type IV pili that mediate adherence to respiratory epithelial and synovial cells. PilA1 is the major pilus subunit in K. kingae type IV pili and is essential for pilus assembly. To develop a better understanding of the role of K. kingae type IV pili during colonization and invasive disease, we examined a collection of clinical isolates for pilus expression and in vitro adherence. In addition, in a subset of isolates we performed nucleotide sequencing to assess the level of conservation of PilA1. The majority of respiratory and nonendocarditis blood isolates were piliated, while the majority of joint fluid, bone, and endocarditis blood isolates were nonpiliated. The piliated isolates formed either spreading/corroding or nonspreadingoncorroding colonies and were uniformly adherent, while the nonpiliated isolates formed domed colonies and were nonadherent. PilA1 sequence varied significantly from strain to strain, resulting in substantial variability in antibody reactivity. These results suggest that type IV pili may confer a selective advantage on K. kingae early in infection and a selective disadvantage on K. kingae at later stages in the pathogenic process. We speculate that PilA1 is immunogenic during natural infection and undergoes antigenic variation to escape the immune response.
机译：Kingella kingae是一种革兰氏阴性细菌，越来越多地被认为是导致儿童败血性关节炎和骨髓炎的原因。先前的工作确定金黄色葡萄球菌表达IV型菌毛，其介导对呼吸道上皮和滑膜细胞的粘附。 PilA1是K. kingae IV型菌毛中主要的菌毛亚基，对于菌毛组装至关重要。为了更好地了解金黄色葡萄球菌IV型菌毛在定植和侵袭性疾病中的作用，我们检查了菌毛表达和体外依从性的临床分离株。另外，在分离株的子集中，我们进行了核苷酸测序以评估PilA1的保守水平。大部分呼吸道和非心内膜炎的血液分离物是有毛的，而大多数关节液，骨骼和心内膜炎的血样是无毛的。毛状分离的菌落形成扩散/腐蚀或不扩散/不腐蚀的菌落，并且均匀粘附，而无毛的分离菌形成圆顶菌落，并且不粘附。 PilA1序列因菌株而异，导致抗体反应性发生显着变化。这些结果表明，IV型菌毛可以在感染的早期赋予金缕梅选择性优势，而在致病过程的后期赋予金缕梅选择性劣势。我们推测，PilA1在自然感染过程中具有免疫原性，并经历抗原变异以逃避免疫反应。

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