• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Probiotic Colonization of the Adherent Mucus Layer of HT29MTXE12 Cells Attenuates Campylobacter jejuni Virulence Properties
【24h】

Probiotic Colonization of the Adherent Mucus Layer of HT29MTXE12 Cells Attenuates Campylobacter jejuni Virulence Properties

机译：HT29MTXE12细胞粘附黏液层的益生菌定植减弱空肠弯曲杆菌的毒力特性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The HT29MTXE12 (E12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. In this study, we have characterized the interaction of Campylobacter jejuni with the E12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate C. jejuni virulence properties. C. jejuni 81-176 colonized and reproduced in E12 mucus. Adhesion to and internalization of C. jejuni were enhanced in E12 cells harboring mucus compared to parental cells without mucus. Translocation of C. jejuni occurred at early time points following infection. C. jejuni aligned with tight junctions and colocalized with the tight junction protein occludin, suggesting a paracellular route of translocation. Probiotic strains Lactobacillus rhamnosus R0011, Lactobacillus helveticus R0052, Lactobacillus salivarius AH102, Bifidobacterium longum AH1205, a commercial combination of L. rhamnosus R0011 and L. helveticus R0052 (Lacidofil), and a cocktail consisting of L. rhamnosus, L. helveticus, and L. salivarius (RhHeSa) colonized E12 mucus and bound to underlying cells. Probiotics attenuated C. jejuni association with and internalization into E12 cells and translocation to the basolateral medium of transwells. Live bacteria and prolonged precolonization of E12 cells with probiotics were necessary for probiotic action. These results demonstrate the potential for E12 cells as a model of mucosal pathogenesis and provide a rationale for the further investigation of probiotics as prophylaxis against human campylobacteriosis.
机译：HT29MTXE12（E12）细胞系带有黏附的黏液层，为体外模拟黏膜感染提供了一种新技术。在这项研究中，我们已经表征了空肠弯曲杆菌与E12细胞系的相互作用，并利用其独特的粘液层来检查益生菌治疗减弱空肠弯曲杆菌毒力特性的潜在功效。空肠弯曲杆菌81-176在E12粘液中定殖并繁殖。与没有粘液的亲代细胞相比，在带有粘液的E12细胞中，空肠弯曲杆菌的粘附和内在化得以增强。空肠弯曲杆菌的移位发生在感染后的早期时间点。空肠弯曲杆菌与紧密连接对齐，并与紧密连接蛋白occludin共定位，提示细胞旁易位。益生菌菌株鼠李糖乳杆菌R0011，瑞士乳杆菌R0052，唾液乳杆菌AH102，长双歧杆菌AH1205，鼠李糖乳杆菌R0011和瑞士乳杆菌R0052（罗西非尔）的商业组合以及由鼠李糖乳杆菌组成的混合物唾液腺（RhHeSa）在E12粘液上定居并与下面的细胞结合。益生菌减弱了空肠弯曲杆菌与E12细胞的结合并内化到E12细胞中，并易位到Transwell的基底外侧培养基中。活细菌和益生菌对E12细胞的长时间定殖对于益生菌作用是必需的。这些结果证明了E12细胞作为粘膜发病机理的模型的潜力，并为进一步研究益生菌作为预防人类弯曲菌病提供了理论依据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号