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首页> 外文期刊>Infection and immunity >T-Cell-Independent Immune Responses Do Not Require Cxc Ligand 13-Mediated B1 Cell Migration
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T-Cell-Independent Immune Responses Do Not Require Cxc Ligand 13-Mediated B1 Cell Migration

机译：T细胞独立的免疫反应不需要Cxc配体13介导的B1细胞迁移。

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The dynamic movement of B cells increases the probability of encountering specific antigen and facilitates cell-cell interactions required for mounting a rapid antibody response. B1a and B1b cells are enriched in the coelomic cavity, contribute to T-cell-independent (TI) antibody responses, and increase in number upon antigen exposure. B1 cell movement is largely governed by Cxc ligand 13 (Cxcl13), and mice deficient in this chemokine have a severe reduction in peritoneal B1 cells. In this study, we examined the role of Cxcl13-dependent B cell migration using Borrelia hermsii infection or intraperitoneal immunization with pneumococcal polysaccharide or 4-hydroxy-3-nitrophenyl-acetyl (NP)-Ficoll, all of which induce robust antibody responses from B1b cells. Surprisingly, we found that antibody responses to B. hermsii or to FhbA, an antigenic target of B1b cells, and the resolution of bacteremia were indistinguishable between wild-type and Cxcl13?/? mice. Importantly, we did not observe an expansion of peritoneal B1b cell numbers in Cxcl13?/? mice. Nonetheless, mice that had resolved infection were resistant to reinfection, indicating that the peritoneal B1b cell reservoir is not required for controlling B. hermsii. Furthermore, despite a reduced peritoneal B1b compartment, immunization with pneumococcal polysaccharide vaccine yielded comparable antigen-specific antibody responses in wild-type and Cxcl13?/? mice and conferred protection against Streptococcus pneumoniae. Likewise, immunization with NP-Ficoll elicited similar antibody responses in wild-type and Cxcl13?/? mice. These data demonstrate that homing of B1 cells into the coelomic cavity is not a requirement for generating protective TI antibody responses, even when antigen is initially localized to this anatomical compartment.
机译：B细胞的动态运动增加了遇到特定抗原的可能性，并促进了快速抗体反应所需的细胞间相互作用。 B1a和B1b细胞富集在腔腔中，有助于T细胞独立（TI）抗体反应，并在抗原暴露后数量增加。 B1细胞的移动主要由Cxc配体13（Cxcl13）控制，缺乏这种趋化因子的小鼠腹膜B1细胞严重减少。在这项研究中，我们研究了使用 Berrelia hermsii 感染或肺炎球菌多糖或4-羟基-3-硝基苯基-乙酰基（NP）-Ficoll进行腹膜内免疫的Cxcl13依赖性B细胞迁移的作用，所有可以诱导来自B1b细胞的强大抗体反应。令人惊讶地，我们发现抗体对 B的应答。 Hermsii 或FhbA（B1b细胞的抗原靶标）和菌血症的消退在野生型和 Cxcl13 ？/？小鼠之间是无法区分的。重要的是，我们没有观察到 Cxcl13 ？/？小鼠腹膜B1b细胞数量的增加。尽管如此，已经解决感染的小鼠对再感染具有抗性，表明控制 B不需要腹膜B1b细胞库。 hermsii 。此外，尽管腹膜B1b区室减少，但肺炎球菌多糖疫苗免疫仍能在野生型和 Cxcl13 ？/？小鼠中产生相当的抗原特异性抗体反应，并赋予了针对< em>肺炎链球菌。同样，NP-Ficoll免疫在野生型和 Cxcl13 ？/？小鼠中引起相似的抗体反应。这些数据表明，即使将抗原最初定位在此解剖区室中，也不需要将B1细胞归巢到腔腔中以产生保护性TI抗体反应。

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