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ygs Is a Novel Gene That Influences Biofilm Formation and the General Stress Response of Staphylococcus epidermidis

机译：ygs是一种新型基因，可影响生物膜的形成和表皮葡萄球菌的一般应激反应

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Infections caused by the nosocomial pathogen Staphylococcus epidermidis frequently develop on implanted medical devices and involve biofilm formation. Biofilms are surface-attached microbial communities that show increased resistance to drug treatment and mechanisms of innate host defense. In this study, a mutant library of the clinical isolate S. epidermidis 1457 was constructed using mariner-based transposon mutagenesis. About a thousand mutants were screened, and 12 mutants were identified as significantly defective in biofilm formation. We focused on a mutant in which the transposon had inserted in a gene with unknown function, SERP0541, which is annotated as a gene encoding a GSP13-like general stress response protein. The gene was named ygs (encoding an unknown general stress protein). Various stresses, including heat, pH, high osmolarity, and ethanol affected the survival of the ygs mutant to a significantly higher degree than the wild-type strain and led to increased expression of ygs. Furthermore, synthesis of polysaccharide intercellular adhesin (PIA) and transcription of the PIA biosynthetic operon were significantly decreased in the ygs mutant. These results are in accordance with the putative involvement of ygs in stress-response gene regulation and indicate that ygs influences biofilm development by controlling PIA-dependent biofilm accumulation. Moreover, ygs had a significant impact on the formation of biofilms and metastatic disease in two catheter-related rat infection models. Our study shows that the ygs gene controls S. epidermidis biofilm accumulation and stress resistance, representing a key regulator of both structural and physiological biofilm characteristics with a significant impact on biofilm-associated infection.
机译：由医院内病原体 Staphylococcus epidermidis 引起的感染经常在植入的医疗设备上发展，并涉及生物膜形成。生物膜是表面附着的微生物群落，显示出对药物治疗的抵抗力增强以及与生俱来的宿主防御机制。在这项研究中，临床分离株 S的突变库。基于水手的转座子诱变构建了表皮1457。筛选了约一千个突变体，并确定了12个突变体在生物膜形成方面存在明显缺陷。我们集中研究了一个转座子插入功能未知的基因 SERP0541 的突变体，该基因被注释为编码GSP13样一般应激反应蛋白的基因。该基因被命名为 ygs （编码未知的一般应激蛋白）。包括热，pH，高渗透压和乙醇在内的各种胁迫对 ygs 突变体的存活的影响程度明显高于野生型菌株，并导致 ygs的表达增加。此外， ygs 突变体的多糖细胞间粘附素（PIA）的合成和PIA生物合成操纵子的转录显着降低。这些结果与 ygs 可能参与的应激反应基因调控一致，表明 ygs 通过控制PIA依赖性生物膜的积累影响生物膜的发育。此外，在两个与导管相关的大鼠感染模型中， ygs 对生物膜的形成和转移性疾病具有重要影响。我们的研究表明， ygs 基因控制着 S。表皮生物膜的积聚和抗逆性，代表着结构和生理生物膜特性的关键调节器，对生物膜相关感染具有重要影响。

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