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Sialic Acid Transport Contributes to Pneumococcal Colonization

机译：唾液酸运输有助于肺炎球菌定植

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Streptococcus pneumoniae is a major cause of pneumonia and meningitis. Airway colonization is a necessary precursor to disease, but little is known about how the bacteria establish and maintain colonization. Carbohydrates are required as a carbon source for pneumococcal growth and, therefore, for colonization. Free carbohydrates are not readily available in the naso-oropharynx; however, N- and O-linked glycans are common in the airway. Sialic acid is the most common terminal modification on N- and O-linked glycans and is likely encountered frequently by S. pneumoniae in the airway. Here we demonstrate that sialic acid supports pneumococcal growth when provided as a sole carbon source. Growth on sialic acid requires import into the bacterium. Three genetic regions have been proposed to encode pneumococcal sialic acid transporters: one sodium solute symporter and two ATP binding cassette (ABC) transporters. Data demonstrate that one of these, satABC, is required for transport of sialic acid. A satABC mutant displayed significantly reduced growth on both sialic acid and the human glycoprotein alpha-1. The importance of satABC for growth on human glycoprotein suggests that sialic acid transport may be important in vivo. Indeed, the satABC mutant was significantly reduced in colonization of the murine upper respiratory tract. This work demonstrates that S. pneumoniae is able to use sialic acid as a sole carbon source and that utilization of sialic acid is likely important during pneumococcal colonization.
机译：肺炎链球菌是引起肺炎和脑膜炎的主要原因。气道定植是疾病必不可少的先兆，但对细菌如何建立和维持定植知之甚少。碳水化合物是肺炎球菌生长和定居的碳源。鼻-口咽中不易获得游离碳水化合物。然而，N-和O-连接的聚糖在气道中很常见。唾液酸是N-和O-连接的聚糖上最常见的末端修饰，肺炎链球菌可能在呼吸道中经常遇到。在这里，我们证明了唾液酸作为唯一碳源提供支持肺炎球菌的生长。唾液酸的生长需要输入细菌中。已经提出了三个遗传区域来编码肺炎球菌唾液酸转运蛋白：一个钠溶质转运蛋白和两个ATP结合盒（ABC）转运蛋白。数据表明，其中之一satABC是唾液酸运输所必需的。 satABC突变体在唾液酸和人糖蛋白α-1上均显示出明显减少的生长。 satABC对人糖蛋白生长的重要性表明唾液酸转运可能在体内很重要。实际上，satABC突变体在鼠上呼吸道的定殖中明显减少。这项工作证明肺炎链球菌能够使用唾液酸作为唯一的碳源，唾液酸的利用在肺炎球菌定植过程中可能很重要。

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