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Gene Discovery in the Freshwater Fish Parasite Trypanosoma carassii: Identification of trans-Sialidase-Like and Mucin-Like Genes

机译:淡水鱼类寄生虫锥虫锥虫的基因发现:反唾液酸酶样和粘蛋白样基因的鉴定

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A total of 1,921 expressed sequence tags (ESTs) were obtained from bloodstream trypomastigotes of Trypanosoma carassii, a parasite of economic importance due to its high prevalence in fish farms. Analysis of the data set allowed us to identify a trans-sialidase (TS)-like gene and three ESTs coding for putative mucin-like genes. TS activity was detected in cell extracts of bloodstream trypomastigotes. We have also used the sequence information obtained to identify genes that have not been previously described in trypanosomatids. (Additional information on these ESTs can be found at http://genoma.unsam.edu.ar/projects/tca.)
机译:从锥虫锥虫的血锥虫中获得了总共1,921个表达的序列标签(EST),锥虫是由于其在鱼类养殖场中的高流行而具有重要经济意义的寄生虫。对数据集的分析使我们能够鉴定出反式唾液酸酶(TS)样基因和编码假定的粘蛋白样基因的三个EST。在血锥虫的细胞提取物中检测到TS活性。我们还使用了获得的序列信息来鉴定锥虫前体中尚未描述的基因。 (有关这些EST的其他信息,请访问http://genoma.unsam.edu.ar/projects/tca。)

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